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Literature summary for 6.1.1.20 extracted from

  • Moor, N.; Linshiz, G.; Safro, M.
    Cloning and expression of human phenylalanyl-tRNA synthetase in Escherichia coli: comparative study of purified recombinant enzymes (2002), Protein Expr. Purif., 24, 260-267.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, individual cloning and coexpression of His-tagged alpha- and beta-subunits in Escherichia coli resulting in 3 different types of (alphabeta)2 heterodimers with the His-tag at the N-terminus, N-terminally His-tagged beta-subunit renders the enzyme inactive, overexpression of nontagged alpha- and beta-subunits in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the N-terminal His-tag does not influence the kinetic parameters of tRNAPhe aminoacylation, cleavage of the His-tag by thrombin leads to nonspecific splitting of the enzyme that occurs in parallel to the main reaction Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
tRNAPhe recombinant heterodimer, pH 8.0, 25°C Homo sapiens
0.00011
-
tRNAPhe recombinant His-tagged heterodimer, pH 8.0, 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
2 * 57000, alpha-subunit, + 2 * 66000, beta-subunit, (alphabeta)2, SDS-PAGE Homo sapiens
66000
-
2 * 57000, alpha-subunit, + 2 * 66000, beta-subunit, (alphabeta)2, SDS-PAGE Homo sapiens
297000
-
recombinant enzyme, dynamic light scattering at 20° Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe Homo sapiens
-
AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged heterodimers and nontagged subunits from Escherichia coli, over 200fold Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe
-
Homo sapiens AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?
ATP + L-phenylalanine + tRNAPhe the N-terminal coiled-coil structure of the alpha-subunit is involved in the binding of cognate tRNAPhe Homo sapiens AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 57000, alpha-subunit, + 2 * 66000, beta-subunit, (alphabeta)2, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
CML33
-
Homo sapiens
FRS
-
Homo sapiens
HSPC173
-
Homo sapiens
L-Phenylalanyl-tRNA synthetase
-
Homo sapiens
Phenylalanine translase
-
Homo sapiens
Phenylalanine--tRNA ligase
-
Homo sapiens
Phenylalanine-tRNA synthetase
-
Homo sapiens
Phenylalanyl transfer ribonucleic acid synthetase
-
Homo sapiens
Phenylalanyl-transfer ribonucleate synthetase
-
Homo sapiens
Phenylalanyl-transfer RNA ligase
-
Homo sapiens
Phenylalanyl-transfer RNA synthetase
-
Homo sapiens
Phenylalanyl-tRNA ligase
-
Homo sapiens
Phenylalanyl-tRNA synthetase
-
Homo sapiens
PheRS
-
Homo sapiens
Synthetase, phenylalanyl-transfer ribonucleate
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens