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Literature summary for 6.1.1.20 extracted from
- Molecular and functional properties of an archaeal phenylalanyl-tRNA synthetase from the hyperthermophile Sulfolobus solfataricus (2002), Biochim. Biophys. Acta, 1596, 246-252.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| elongation factor 1alpha | interaction enhances the activity | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00048 | - |
L-phenylalanine | pH 7.8, 60°C | Saccharolobus solfataricus |  |
| 0.0015 | - |
L-phenylalanine | pH 7.8, 70°C | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
2 * 56000, alpha-subunit, + 2 * 64000, beta-subunit, (alphabeta)2, SDS-PAGE | Saccharolobus solfataricus |
| 64000 | - |
2 * 56000, alpha-subunit, + 2 * 64000, beta-subunit, (alphabeta)2, SDS-PAGE | Saccharolobus solfataricus |
| 240000 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | Saccharolobus solfataricus | enzyme is essemtial for poly(Phe) synthesis | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 60fold | Saccharolobus solfataricus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe | tRNA channeling mechanism | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | activity is restricted to endogenous tRNA, highly specific for L-phenylalanine | Saccharolobus solfataricus | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
| ATP + L-phenylalanine + tRNAPhe | enzyme is essemtial for poly(Phe) synthesis | Saccharolobus solfataricus | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
| GTP + L-phenylalanine + tRNAPhe | 40fold lower activity compared to ATP | Saccharolobus solfataricus | GMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 56000, alpha-subunit, + 2 * 64000, beta-subunit, (alphabeta)2, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CML33 | - |
Saccharolobus solfataricus |
| FRS | - |
Saccharolobus solfataricus |
| HSPC173 | - |
Saccharolobus solfataricus |
| L-Phenylalanyl-tRNA synthetase | - |
Saccharolobus solfataricus |
| Phenylalanine translase | - |
Saccharolobus solfataricus |
| Phenylalanine--tRNA ligase | - |
Saccharolobus solfataricus |
| Phenylalanine-tRNA synthetase | - |
Saccharolobus solfataricus |
| Phenylalanyl transfer ribonucleic acid synthetase | - |
Saccharolobus solfataricus |
| Phenylalanyl-transfer ribonucleate synthetase | - |
Saccharolobus solfataricus |
| Phenylalanyl-transfer RNA ligase | - |
Saccharolobus solfataricus |
| Phenylalanyl-transfer RNA synthetase | - |
Saccharolobus solfataricus |
| Phenylalanyl-tRNA ligase | - |
Saccharolobus solfataricus |
| Phenylalanyl-tRNA synthetase | - |
Saccharolobus solfataricus |
| PheRS | - |
Saccharolobus solfataricus |
| Synthetase, phenylalanyl-transfer ribonucleate | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 92 | - |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 88 | - |
10 min, 50% inactivation | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
L-phenylalanine | pH 7.8, 60°C | Saccharolobus solfataricus | |
| 0.115 | - |
L-phenylalanine | pH 7.8, 70°C | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
broad maximum | Saccharolobus solfataricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | not dependent on | Saccharolobus solfataricus | |
| GTP | 40fold lower activity than with ATP | Saccharolobus solfataricus | |
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