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Literature summary for 6.1.1.20 extracted from

  • Rauhut, R.; Gabius, H.J.; Cramer, F.
    Phenylalanyl-tRNA synthetase from chloroplast of a higher plant (Phaseolus vulgaris). Purification and comparison of its structural, functional, and immunological properties with those of the enzymes from the corresponding cytoplasm, the cyanobacterium Anacystis nidulans, and the photosynthetic green sulfur bacterium Chlorobium limicola (1986), J. Biol. Chem., 261, 2799-2803.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Phaseolus vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Phaseolus vulgaris 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
1 * 33000 (alpha) + 1 * 42000 (beta), SDS-PAGE Phaseolus vulgaris
42000
-
1 * 33000 (alpha) + 1 * 42000 (beta), SDS-PAGE Phaseolus vulgaris
78000
-
gel filtration Phaseolus vulgaris
260000
-
-
Phaseolus vulgaris
260000
-
gel filtration Synechococcus elongatus PCC 7942 = FACHB-805
260000
-
gel filtration Chlorobium limicola

Organism

Organism UniProt Comment Textmining
Chlorobium limicola
-
-
-
Phaseolus vulgaris
-
-
-
Synechococcus elongatus PCC 7942 = FACHB-805
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0107
-
-
Chlorobium limicola
0.092
-
-
Synechococcus elongatus PCC 7942 = FACHB-805
0.11
-
-
Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe
-
Phaseolus vulgaris AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?
ATP + L-phenylalanine + tRNAPhe
-
Synechococcus elongatus PCC 7942 = FACHB-805 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?
ATP + L-phenylalanine + tRNAPhe
-
Chlorobium limicola AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?

Subunits

Subunits Comment Organism
dimer 1 * 33000 (alpha) + 1 * 42000 (beta), SDS-PAGE Phaseolus vulgaris