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Literature summary for 6.1.1.20 extracted from
- Purification and reversible subunit dissociation of overproduced Escherichia coli phenylalanyl-tRNA synthetase (1983), Biochim. Biophys. Acta, 741, 244-250.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 98000 (alpha) + 2 * 38000 (beta), SDS-PAGE | Escherichia coli |
| 98000 | - |
2 * 98000 (alpha) + 2 * 38000 (beta), SDS-PAGE | Escherichia coli |
| 250000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
overproducing strain carrying a hybrid pBR322 plasmid containing the pheS-pheT locus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.8 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | - |
Escherichia coli | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 98000 (alpha) + 2 * 38000 (beta), SDS-PAGE | Escherichia coli |
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Release 2023.1 (January 2023)
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