Cloned (Comment) | Organism |
---|---|
gene trpRS, expression of His-tagged TrpRS in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged TrpRS in apo form and in complexes with AMP, Trp, or TrpNH2O, the complexes contain a sulfate ion bound at the active site which mimics the alpha- or beta-phosphate group of ATP during tryptophan activation, hanging drop vapor diffusion method, 20°C, 15 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 5 mM 2-mercaptoethanol, and 1 mM PMSF, mixed with crystallization solutions, containing 0.1 M HEPES, pH 5.9, and 2.2 M (NH4)2SO4, with 0.0005 ml of 20 mM Trp or TrpNH2O for the Trp or TrpNH2O complexes, or with 100 mM ATP for the AMP complex, 2 days to 1 week, X-ray diffraction structure determination and analysis at 2.1-3.1 A resolution, modelling | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene trpS | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged TrpRS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
ATP + L-tryptophan + tRNATrp | Trp and AMP binding structures, overview | Saccharomyces cerevisiae | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure molecular modeling and dynamics simulation, overview | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
TrpRS | - |
Saccharomyces cerevisiae |
Tryptophanyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | specific activation of amino acids by aminoacyl-tRNA synthetases is essential for maintaining translational fidelity | Saccharomyces cerevisiae |