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Literature summary for 6.1.1.2 extracted from
- Crystal structure of Pyrococcus horikoshii tryptophanyl-tRNA synthetase and structure-based phylogenetic analysis suggest an archaeal origin of tryptophanyl-tRNA synthetase (2010), Nucleic Acids Res., 38, 1401-1412.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the trpS1 gene is inserted into the vector pET28a for expression in Escherichia coli BL21DE3 cells | Pyrococcus horikoshii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the structure of tryptophanyl-tRNA synthetase in complex with tryptophanyl-5' AMP is solved at 3.0 A resolution | Pyrococcus horikoshii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | Pyrococcus horikoshii | - |
AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | O59584 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| on a Ni-affinity column | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | - |
Pyrococcus horikoshii | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrpRS | - |
Pyrococcus horikoshii |
| Tryptophanyl-tRNA synthetase | - |
Pyrococcus horikoshii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Pyrococcus horikoshii | |
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Release 2023.1 (January 2023)
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