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Literature summary for 6.1.1.2 extracted from

  • Tsuchiya, W.; Hasegawa, T.
    Molecular recognition of tryptophan tRNA by tryptophanyl-tRNA synthetase from Aeropyrum pernix K1 (2009), J. Biochem., 145, 635-641.
    View publication on PubMed
Search for more literature for 6.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
the trpS gene is cloned into the pET30a+ vector for expression in Escherichia coli BL21-CodonPlus DE3-RIL cells Aeropyrum pernix K1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 tRNATrp
-
 Aeropyrum pernix K1
0.0017
-
 tRNATrp(A73C) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.0019
-
 tRNATrp(G2C-G71G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.0021
-
 tRNAArg pH 7.5, 60°C Aeropyrum pernix K1
0.0051
-
 tRNATrp(A73U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.0053
-
 tRNATrp(A36G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.0055
-
 tRNATrp(G1C-G72G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.0058
-
 tRNATrp(G2U-C71A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.0058
-
 tRNATrp(A73G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.0058
-
 tRNATrp(A36U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.0059
-
 tRNATrp(A36C) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.006
-
 tRNATrp(HOG1) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.0098
-
 tRNATrp(A36U, A73G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.012
-
 tRNATrp(pppG1) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.014
-
 tRNATrp(C35A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.015
-
 tRNATrp(G2A-C71U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.021
-
 tRNATrp(G1U-C72A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.021
-
 tRNATrp(C35U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.025
-
 tRNATrp(G1A-C72U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.038
-
 tRNATrp(C34G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.038
-
 tRNATrp(C35G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.048
-
 tRNATrp(C34U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.06
-
 tRNATrp(C34A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tryptophan + tRNATrp Aeropyrum pernix K1
-
 AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix K1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography using a Cibacron Blue 3GA column Aeropyrum pernix K1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tryptophan + tRNAArg
-
 Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNAArg
-
 ?
ATP + L-tryptophan + tRNATrp
-
 Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?
ATP + L-tryptophan + tRNATrp(A36C) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A36C)
-
 ?
ATP + L-tryptophan + tRNATrp(A36G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A36G)
-
 ?
ATP + L-tryptophan + tRNATrp(A36U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A36U)
-
 ?
ATP + L-tryptophan + tRNATrp(A36U, A73G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A36U, A73G)
-
 ?
ATP + L-tryptophan + tRNATrp(A73C) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A73C)
-
 ?
ATP + L-tryptophan + tRNATrp(A73G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A73G)
-
 ?
ATP + L-tryptophan + tRNATrp(A73U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A73U)
-
 ?
ATP + L-tryptophan + tRNATrp(C34A) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C34A)
-
 ?
ATP + L-tryptophan + tRNATrp(C34G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C34G)
-
 ?
ATP + L-tryptophan + tRNATrp(C34U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C34U)
-
 ?
ATP + L-tryptophan + tRNATrp(C35A) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C35A)
-
 ?
ATP + L-tryptophan + tRNATrp(C35G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C35G)
-
 ?
ATP + L-tryptophan + tRNATrp(C35U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C35U)
-
 ?
ATP + L-tryptophan + tRNATrp(G1A-C72U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G1A-C72U)
-
 ?
ATP + L-tryptophan + tRNATrp(G1C-G72G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G1C-G72G)
-
 ?
ATP + L-tryptophan + tRNATrp(G1U-C72A) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G1U-C72A)
-
 ?
ATP + L-tryptophan + tRNATrp(G2A-C71U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G2A-C71U)
-
 ?
ATP + L-tryptophan + tRNATrp(G2C-G71G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G2C-G71G)
-
 ?
ATP + L-tryptophan + tRNATrp(G2U-C71A) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G2U-C71A)
-
 ?
ATP + L-tryptophan + tRNATrp(HOG1) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(HOG1)
-
 ?
ATP + L-tryptophan + tRNATrp(pppG1) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(pppG1)
-
 ?

Synonyms

Synonyms Comment Organism
TrpRS
-
 Aeropyrum pernix K1
Tryptophanyl-tRNA synthetase
-
 Aeropyrum pernix K1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 tryptophanylation assay Aeropyrum pernix K1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.079
-
 tRNATrp(C35G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.11
-
 tRNATrp(C34U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.13
-
 tRNATrp(C34G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.15
-
 tRNATrp(C35A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.16
-
 tRNAArg pH 7.5, 60°C Aeropyrum pernix K1
0.17
-
 tRNATrp(C34A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.27
-
 tRNATrp(A36U, A73G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.34
-
 tRNATrp(G1C-G72G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.46
-
 tRNATrp(G1U-C72A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.47
-
 tRNATrp(C35U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
0.62
-
 tRNATrp(A73G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
1.7
-
 tRNATrp(G2C-G71G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
2.3
-
 tRNATrp(G1A-C72U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
3.2
-
 tRNATrp(HOG1) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
4.4
-
 tRNATrp(A36G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
4.5
-
 tRNATrp(pppG1) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
5.5
-
 tRNATrp(A73C) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1
-
 tRNATrp(A73U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
7.2
-
 tRNATrp(G2U-C71A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
11
-
 tRNATrp(A36C) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
16
-
 tRNATrp pH 7.5, 60°C Aeropyrum pernix K1
19
-
 tRNATrp(G2A-C71U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
21
-
 tRNATrp(A36U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 tryptophanylation assay Aeropyrum pernix K1

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Aeropyrum pernix K1