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Literature summary for 6.1.1.2 extracted from

  • Wakasugi, K.
    An exposed cysteine residue of human angiostatic mini tryptophanyl-tRNA synthetase (2010), Biochemistry, 49, 3156-3160.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
heme enhances activity Homo sapiens

Application

Application Comment Organism
medicine human mini, but not full-length, TrpRS has angiostatic activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cDNA fragments of human full-length TrpRS, aa 1-471, mini TrpRS, aa 48-471, and T1 TrpRS, aa 71-471, are separately cloned into the pET20b vector for expression in Escherichia coli BL21DE3 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H130R full-length H130R TrpRS is constitutively active, the mini H130R TrpRS mutant forms disulfide bonds and there are additional functional differences between the full-length and mini forms Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ enhances activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tryptophan + tRNATrp Homo sapiens
-
AMP + diphosphate + L-tryptophyl-tRNATrp
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P23381
-
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant six-His-tagged proteins are purified on a nickel affinity column Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tryptophan + tRNATrp
-
Homo sapiens AMP + diphosphate + L-tryptophyl-tRNATrp
-
?

Synonyms

Synonyms Comment Organism
mini tryptophanyl-tRNA synthetase truncated form Homo sapiens
TrpRS
-
Homo sapiens
Tryptophanyl-tRNA synthetase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
aminoacylation assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
aminoacylation assay Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens