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Literature summary for 6.1.1.2 extracted from

  • Vecchione, J.J.; Sello, J.K.
    A novel tryptophanyl-tRNA synthetase gene confers high-level resistance to indolmycin (2009), Antimicrob. Agents Chemother., 53, 3972-3980.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.2

Application

Application Comment Organism
medicine as producers of nearly half of the 10000 known antibiotics, Streptomyces bacteria have been particularly important in drug discovery and are a major source of drug resistance genes Streptomyces coelicolor
medicine as producers of nearly half of the 10000 known antibiotics, Streptomyces bacteria have been particularly important in drug discovery and are a major source of drug resistance genes Streptomyces griseus

Cloned(Commentary)

Cloned (Comment) Organism
the vectors pMS81, pIJ10257, and pBluescript KS+ are used Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
TrpRS2(H48N) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
TrpRS2(H48Q) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
TrpRS2(L9F) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
TrpRS2(L9F/H48Q) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
TrpRS2(L9F/Q13K) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
TrpRS2(Q13K) ORF mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
TrpRS2[C(-17)A/H48Q] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
TrpRS2[C(-17)A/L9F] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
TrpRS2[C(-17)A] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
TrpRS2[G(-13)T/H48Q] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
TrpRS2[G(-13)T/L9F] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor
TrpRS2[G(-13)T] locus mutant, constructed for analysing the indolmycin resistance Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
additional information indolmycin, a potential antibacterial drug, completely inhibits bacterial tryptophanyl-tRNA synthetases, identifying indolmycin resistance genes leads to the discovery of a gene encoding an indolmycin-resistant isoform of tryptophanyl-tRNA synthetase Streptomyces coelicolor
additional information indolmycin, a potential antibacterial drug, completely inhibits bacterial tryptophanyl-tRNA synthetases, identifying indolmycin resistance genes leads to the discovery of a gene encoding an indolmycin-resistant isoform of tryptophanyl-tRNA synthetase Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tryptophan + tRNATrp Streptomyces coelicolor
-
 AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?
ATP + L-tryptophan + tRNATrp Streptomyces griseus
-
 AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces griseus B1VQY7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tryptophan + tRNATrp
-
 Streptomyces coelicolor AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?
ATP + L-tryptophan + tRNATrp
-
 Streptomyces griseus AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?

Synonyms

Synonyms Comment Organism
TrpRS2
-
 Streptomyces coelicolor
TrpRS2
-
 Streptomyces griseus
Tryptophanyl-tRNA synthetase
-
 Streptomyces coelicolor
Tryptophanyl-tRNA synthetase
-
 Streptomyces griseus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Streptomyces coelicolor
ATP
-
 Streptomyces griseus