Cloned (Comment) | Organism |
---|---|
gene trpS, enzyme expression in Escherichia coli strain BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
I311E | mutation located at the appended beta1-beta2 hairpin and the AIDQ sequence of TrpRS that switch this enzyme to a tRNA-dependent mode in the tryptophan activation step | Homo sapiens |
I311V | mutation located at the appended beta1-beta2 hairpin and the AIDQ sequence of TrpRS that switch this enzyme to a tRNA-dependent mode in the tryptophan activation step | Homo sapiens |
V85A | mutation located at the appended beta1-beta2 hairpin and the AIDQ sequence of TrpRS that switch this enzyme to a tRNA-dependent mode in the tryptophan activation step, the mutant shows decreased, but visible tryptophan activation activity in the ATP-diphosphate exchange reaction | Homo sapiens |
V85A/V90A | mutation located at the appended beta1-beta2 hairpin and the AIDQ sequence of TrpRS that switch this enzyme to a tRNA-dependent mode in the tryptophan activation step | Homo sapiens |
V85E | mutation located at the appended beta1-beta2 hairpin and the AIDQ sequence of TrpRS that switch this enzyme to a tRNA-dependent mode in the tryptophan activation step | Homo sapiens |
V85K | mutation located at the appended beta1-beta2 hairpin and the AIDQ sequence of TrpRS that switch this enzyme to a tRNA-dependent mode in the tryptophan activation step, the V85K mutant has barely detectable aminoacylation activity | Homo sapiens |
V85L | mutation located at the appended beta1-beta2 hairpin and the AIDQ sequence of TrpRS that switch this enzyme to a tRNA-dependent mode in the tryptophan activation step, the V85L mutant is able to acylate bovine tRNATrp with very high effciency | Homo sapiens |
V85S | mutation located at the appended beta1-beta2 hairpin and the AIDQ sequence of TrpRS that switch this enzyme to a tRNA-dependent mode in the tryptophan activation step, the mutant shows no activity in the ATP-diphosphate exchange reaction, but retains aminoacylation activity | Homo sapiens |
V90A | mutation located at the appended beta1-beta2 hairpin and the AIDQ sequence of TrpRS that switch this enzyme to a tRNA-dependent mode in the tryptophan activation step, the mutant shows decreased, but visible tryptophan activation activity in the ATP-diphosphate exchange reaction, the V90 mutation enhances the hydrophobic interaction between V85 and I311 | Homo sapiens |
V90S | mutation located at the appended beta1-beta2 hairpin and the AIDQ sequence of TrpRS that switch this enzyme to a tRNA-dependent mode in the tryptophan activation step, the mutant shows decreased, but visible tryptophan activation activity in the ATP-diphosphate exchange reaction, the V90 mutation enhances the hydrophobic interaction between V85 and I311 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00029 | - |
tRNATrp | pH 7.5, 30°C, mutant V85K | Homo sapiens | |
0.0013 | - |
tRNATrp | pH 7.5, 30°C, wild-type enzyme | Homo sapiens | |
0.00141 | - |
tRNATrp | pH 7.5, 30°C, mutant V85A | Homo sapiens | |
0.00184 | - |
tRNATrp | pH 7.5, 30°C, mutant V90A | Homo sapiens | |
0.00214 | - |
tRNATrp | pH 7.5, 30°C, mutant V85S | Homo sapiens | |
0.00223 | - |
tRNATrp | pH 7.5, 30°C, mutant V85A/V90A | Homo sapiens | |
0.0052 | - |
tRNATrp | pH 7.5, 30°C, mutant V90S | Homo sapiens | |
0.0085 | - |
tRNATrp | pH 7.5, 30°C, mutant V85E | Homo sapiens | |
0.0248 | - |
tRNATrp | pH 7.5, 30°C, mutant V85L | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Homo sapiens | - |
AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P23381 | gene trpS | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Homo sapiens | AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? | |
ATP + L-tryptophan + tRNATrp | bovine tRNATrp substrate, a two step reaction, the tryptophanyl-tRNA synthetase is a class I amino acid tRNA-synthetase, that catalyzes tryptophan activation in the absence of its cognate tRNA, cognate tRNA is not obligatory to catalyze amino acid activation, the integrated 3 end of the tRNA is necessary to activate the ATP-diphosphate exchange reaction, overview | Homo sapiens | AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | primary and tertiary structure of human TrpRS, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TrpRS | - |
Homo sapiens |
Tryptophanyl-tRNA synthetase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
tRNATrp | pH 7.5, 30°C, mutant V85K | Homo sapiens | |
0.02 | - |
tRNATrp | pH 7.5, 30°C, mutant V85E | Homo sapiens | |
0.028 | - |
tRNATrp | pH 7.5, 30°C, mutant V85S | Homo sapiens | |
0.047 | - |
tRNATrp | pH 7.5, 30°C, mutant V85A/V90A | Homo sapiens | |
0.063 | - |
tRNATrp | pH 7.5, 30°C, mutant V90S | Homo sapiens | |
0.48 | - |
tRNATrp | pH 7.5, 30°C, mutant V85A | Homo sapiens | |
1.37 | - |
tRNATrp | pH 7.5, 30°C, wild-type enzyme | Homo sapiens | |
1.39 | - |
tRNATrp | pH 7.5, 30°C, mutant V90A | Homo sapiens | |
3.3 | - |
tRNATrp | pH 7.5, 30°C, mutant V85L | Homo sapiens | |
5.11 | - |
tRNATrp | pH 7.5, 30°C, mutant V85L | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |