Crystallization (Comment) | Organism |
---|---|
purified protein in complex with uncharged tRNA and aminoacylated tRNA in a 1:1 mixture, sitting drop vapour diffusion method, 0.002 ml of protein in 10 mM HEPES, pH 7.5, 20 mM KCl, 0.02% NaN3, and 2 mM 2-mercaptoethanol, addition of 230 mM of TrpRS, 250 mM of tRNATrp, 5 mM tryptophan and 10 mM AMP-PNP, mixed with 0.002 ml reservoir solution containing 2 M ammonium sulfate and 0.1 M HEPES pH 6.9, 4°C, X-ray diffraction structure determination and analysis at 2.9 A resolution, SAD method using a selenium-labeled crystal | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | preparation of alpha17 deletion mini-TrpRS, DELTAD382Q389, by site-directed mutagensis, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Homo sapiens | - |
AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P23381 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Homo sapiens | AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? | |
ATP + L-tryptophan + tRNATrp | tRNA substrate from Bos taurus, tryptophan binding pocket structure, overview, two distinct tRNA conformations: uncharged tRNA is bound across the dimer, with anticodon and acceptor stem interacting with separate subunits, in this cross-dimer tRNA complex, the class I enzyme has a class II-like tRNA binding mode, the aminoacylated tRNA is bound only by the anticodon, the acceptor stem being free and having space to interact precisely with EF-1a, suggesting that the product of aminoacylation can be directly handed off to EF-1alpha for the next step of protein synthesis, recognition mechanisms, overview | Homo sapiens | AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? | |
additional information | the enzyme interacts directly with elongation factor 1alpha, which carries charged tRNA to the ribosome | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure determination and analysis, sequence comparisons, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TrpRS | - |
Homo sapiens |
Tryptophanyl-tRNA synthetase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at, aminoacylation | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, aminoacylation | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |