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Literature summary for 6.1.1.2 extracted from
- Human tryptophanyl-tRNA synthetase binds with heme to enhance its aminoacylation activity (2007), Biochemistry, 46, 11291-11298.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| hemin | heme can interact strongly with Zn2+-depleted full-length TrpRS, but not the Zn2+-bound enzyme, with a stoichiometric heme:protein ratio of 1:1 to enhance the aminoacylation activity significantly, involvement of heme in regulation of TrpRS aminoacylation activity, overview | Homo sapiens |  |
| additional information | TrpRS is highly upregulated by interferon-gamma, IFN-gamma | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H129A | site-directed mutagenesis, hemin binding capacity of the mutant enzymes compared to the wild-type enzyme, overview | Homo sapiens |
| H130R | site-directed mutagenesis, the Zn2+-unbound mutant cannot bind heme in contrast to the Zn2+-free wild-type enzyme | Homo sapiens |
| H140A | site-directed mutagenesis, hemin binding capacity of the mutant enzymes compared to the wild-type enzyme, overview | Homo sapiens |
| H170A | site-directed mutagenesis, hemin binding capacity of the mutant enzymes compared to the wild-type enzyme, overview | Homo sapiens |
| H173A | site-directed mutagenesis, hemin binding capacity of the mutant enzymes compared to the wild-type enzyme, overview | Homo sapiens |
| H257A | site-directed mutagenesis, hemin binding capacity of the mutant enzymes compared to the wild-type enzyme, overview | Homo sapiens |
| H336A | site-directed mutagenesis, hemin binding capacity of the mutant enzymes compared to the wild-type enzyme, overview | Homo sapiens |
| H375A | site-directed mutagenesis, hemin binding capacity of the mutant enzymes compared to the wild-type enzyme, overview | Homo sapiens |
| H387A | site-directed mutagenesis, hemin binding capacity of the mutant enzymes compared to the wild-type enzyme, overview | Homo sapiens |
| H445A | site-directed mutagenesis, hemin binding capacity of the mutant enzymes compared to the wild-type enzyme, overview | Homo sapiens |
| H73A | site-directed mutagenesis, hemin binding capacity of the mutant enzymes compared to the wild-type enzyme, overview | Homo sapiens |
| additional information | construction of truncated enzyme variants, hemin binding capacities, overview | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| succinylacetone | a heme biosynthesis inhibitor, inhibit cellular TrpRS activity in IFN-gamma-activated cells without affecting TrpRS protein expression | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
| additional information | no effects by Fe2+ and diphosphate | Homo sapiens | |
| Zn2+ | TrpRS is a Zn2+-binding protein, highly activates the enzyme, the activation effect is reduced by protoporphyrin IX, overview | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | Homo sapiens | involvement of heme in regulation of TrpRS aminoacylation activity | AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P23381 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, detagged enzyme by anion exchange chromatography, gel filtration, and dialysis | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | involvement of heme in regulation of TrpRS aminoacylation activity | Homo sapiens | AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? | |
| ATP + L-tryptophan + tRNATrp | tRNA substrate from Saccharomyces cerevisiae | Homo sapiens | AMP + diphosphate + L-tryptophanyl-tRNATrp | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrpRS | - |
Homo sapiens |
| Tryptophanyl-tRNA synthetase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
about, aminoacylation assay at room temperature | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
aminoacylation assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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