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Literature summary for 6.1.1.2 extracted from

  • Kisselev, L.L.; Favorova, O.O.; Kovaleva, G.K.
    Tryptophanyl-tRNA synthetase from beef pancreas (1979), Methods Enzymol., 54, 234-257.
No PubMed abstract available
Search for more literature for 6.1.1.2

General Stability

General Stability Organism
not markedly inactivated by repeated freezing and thawing Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
2'-O-methyladenosine
-
 Bos taurus
3'-O-methyl-ATP ATP-diphosphate exchange, noncompetitive with respect to ATP Bos taurus
3'-O-Methyladenosine ATP-diphosphate exchange, noncompetitive with respect to ATP Bos taurus
4,5,6,7-Tetrafluorotryptophan
-
 Bos taurus
5,7-Difluorotryptophan
-
 Bos taurus
5-hydroxytryptophan ATP-diphosphate exchange, weak Bos taurus
5-Methyltryptophan ATP-diphosphate exchange, weak Bos taurus
6-methyltryptophan ATP-diphosphate exchange, weak Bos taurus
9-(2',3'-Dihydroxypropyl)adenine S-isomer Bos taurus
9-(2'-Hydroxyethyl)adenine
-
 Bos taurus
9-(2'-Hydroxyethyl)adenine triphosphate
-
 Bos taurus
9-(3'-Hydroxypropyl)adenine
-
 Bos taurus
9-(3'-Hydroxypropyl)adenine triphosphate
-
 Bos taurus
9-(4'-Hydroxybutyl)adenine
-
 Bos taurus
9-(4'-Hydroxybutyl)adenine triphosphate
-
 Bos taurus
adenine
-
 Bos taurus
adenosine
-
 Bos taurus
Adenosine-5'-(beta-bromoethane phosphonate)
-
 Bos taurus
Adenosine-5'-(beta-bromoethanediphosphonate)
-
 Bos taurus
Adenosine-5'-(beta-chloroethyl phosphate)
-
 Bos taurus
Adenosine-5'-chloromethylphosphonate ATP-diphosphate exchange, reversible, incompetitive Bos taurus
alpha,beta-methylene-ATP
-
 Bos taurus
beta-Indolyl propionic acid
-
 Bos taurus
beta-indolylacetic acid
-
 Bos taurus
beta-Indolylpyruvic acid
-
 Bos taurus
beta-Methyl-DL-tryptophan ATP-diphosphate exchange Bos taurus
D-tryptophan
-
 Bos taurus
diethyl dicarbonate substrates protect, completely reactivated by hydroxylamine Bos taurus
DL-Tryptophanyl hydroxamate ATP-diphosphate exchange Bos taurus
gamma-(n-Azidoanilide)-ATP
-
 Bos taurus
Mixed anhydride of ADP and mesithylenecarboxylic acid
-
 Bos taurus
Mixed anhydride of AMP and mesithylenecarboxylic acid
-
 Bos taurus
N-acetyl-L-tryptophan
-
 Bos taurus
N-Formyl-L-tryptophan
-
 Bos taurus
NEM
-
 Bos taurus
tryptamine
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00012
-
 tRNATrp aminoacylation Bos taurus
0.5
-
 ATP diphosphate, tryptophan-dependent ATP-diphosphate exchange Bos taurus
2
-
 ATP tryptophan-dependent ATP-diphosphate exchange Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE Bos taurus
120000
-
 gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.45
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-10°C, in 30% ethylene glycol, stable for at least 6 months Bos taurus
-70°C, stable for at least 6 months Bos taurus
4°C, enzyme concentration 0.5-4 mg/ml in 10 mM TrisHCl buffer, pH 7.5, 0.1 mM EDTA, 1 mM DTT, without tryptophan, stable for 1 week Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tryptophan + tRNATrp does not activate any natural amino acid except L-tryptophan Bos taurus AMP + diphosphate + L-tryptophan-tRNATrp
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 60000, SDS-PAGE Bos taurus