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Literature summary for 6.1.1.19 extracted from

  • Zhang, Q.S.; Shen, L.; Wang, E.D.; Wang, Y.L.
    Biosynthesis and characterization of 4-fluorotryptophan-labeled Escherichia coli arginyl-tRNA synthetase (1999), J. Protein Chem., 18, 187-192.
    View publication on PubMed
Search for more literature for 6.1.1.19

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information biosynthetic preparation of an enzyme where Trp are replaced by 4-fluorotryptophane Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 tRNAArg aminoacylation reaction Escherichia coli
0.003
-
 tRNAArg aminoacylation reaction, 4-fluorotryptophan-labeled enzyme Escherichia coli
0.012
-
 L-arginine aminoacylation reaction Escherichia coli
0.0137
-
 L-arginine aminoacylation reaction, 4-fluorotryptophan-labeled enzyme Escherichia coli
0.9
-
 ATP aminoacylation reaction Escherichia coli
1.35
-
 ATP aminoacylation reaction, 4-fluorotryptophan-labeled enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 incorporation of 4-fluorotryptophan has little effect on activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine + tRNAArg
-
 Escherichia coli AMP + diphosphate + L-arginyl-tRNAArg
-
 ?