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Literature summary for 6.1.1.19 extracted from
- Arginyl-tRNA synthetase with signature sequence KMSK from Bacillus stearothermophilus (2003), Biochem. J., 376, 773-779.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli of a His-tagged recombinant enzyme, the enzyme has 73% homology with respect to Bacillus subtilis ArgRS, and 22% with respect to Escherichia coli | Geobacillus stearothermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G136K | inactive enzyme | Geobacillus stearothermophilus |
| K382A | half as active as the native enzyme | Geobacillus stearothermophilus |
| K385A | inactive enzyme | Geobacillus stearothermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
L-arginine | ATP-diphosphate exchange reaction, pH 8, 55°C | Geobacillus stearothermophilus |  |
| 0.0017 | - |
tRNAArg | aminoacylation reaction, pH 8, 37°C | Geobacillus stearothermophilus | |
| 0.0029 | - |
tRNAArg | aminoacylation reaction, pH 8, 55°C | Geobacillus stearothermophilus | |
| 0.0057 | - |
L-arginine | aminoacylation reaction, pH 8, 37°C | Geobacillus stearothermophilus | |
| 0.0074 | - |
L-arginine | ATP-diphosphate exchange reaction, pH 8, 37°C | Geobacillus stearothermophilus | |
| 0.0094 | - |
L-arginine | aminoacylation reaction, pH 8, 55°C | Geobacillus stearothermophilus | |
| 1 | - |
ATP | aminoacylation reaction, pH 8, 55°C | Geobacillus stearothermophilus | |
| 1.29 | - |
ATP | ATP-diphosphate exchange reaction, pH 8, 55°C | Geobacillus stearothermophilus | |
| 1.3 | - |
ATP | aminoacylation reaction, pH 8, 37°C | Geobacillus stearothermophilus | |
| 1.31 | - |
ATP | ATP-diphosphate exchange reaction, pH 8, 37°C | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62800 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of a His-tagged recombinant enzyme using Ni-nitrilotriacetic acid agarose affinity chromatography | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-arginine + tRNAArg | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62800, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
activity three-fold higher than at 37°C | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
stable for 10 min | Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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