Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Geobacillus stearothermophilus | |
Periodate-oxidized tRNA | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | 0.0006 | tRNAArg | pH 7.4 | Geobacillus stearothermophilus | |
0.001 | 0.002 | Arg | - |
Geobacillus stearothermophilus | |
1 | - |
ATP | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
gel filtration, sucrose density gradient centrifugation | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus NCA 1518 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-arginine + tRNAArg = AMP + diphosphate + L-arginyl-tRNAArg | mechanism includes the formation of an enzyme-bound aminoacyl-adenylate as an intermediate in the first step followed by transfer of the amino acid to tRNA | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
ATP + L-arginine + tRNAArg | - |
Geobacillus stearothermophilus NCA 1518 | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 59000, SDS-PAGE | Geobacillus stearothermophilus |
More | cognate tRNA induces dimerization | Geobacillus stearothermophilus |