General Stability | Organism |
---|---|
not affected by four freeze-thaw cycles | Saccharomyces pastorianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
tRNAArgIII | aminoacylation | Saccharomyces pastorianus | |
0.0015 | - |
Arg | aminoacylation | Saccharomyces pastorianus | |
0.0025 | - |
tRNAArg | Arg, , ATP-diphosphate exchange | Saccharomyces pastorianus | |
0.05 | - |
diphosphate | ATP-diphosphate exchange | Saccharomyces pastorianus | |
0.3 | - |
ATP | aminoacylation | Saccharomyces pastorianus | |
1.4 | - |
ATP | ATP-diphosphate exchange | Saccharomyces pastorianus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgCl2, optimal concentration in aminoacylation: 7 mM , when ATP concentration is 10 mM | Saccharomyces pastorianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration | Saccharomyces pastorianus |
70000 | 73000 | sucrose density gradient centrifugation | Saccharomyces pastorianus |
74000 | - |
1 * 74000, SDS-PAGE | Saccharomyces pastorianus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces pastorianus | - |
- |
- |
Saccharomyces pastorianus 836 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
large-scale | Saccharomyces pastorianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Saccharomyces pastorianus | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
ATP + L-arginine + tRNAArg | - |
Saccharomyces pastorianus 836 | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
ATP + L-arginine + tRNAArgIII | - |
Saccharomyces pastorianus | AMP + diphosphate + L-arginyl-tRNAArgIII | - |
? | |
ATP + L-arginine + tRNAArgIII | - |
Saccharomyces pastorianus 836 | AMP + diphosphate + L-arginyl-tRNAArgIII | - |
? | |
additional information | arginine-dependent ATP-diphosphate exchange | Saccharomyces pastorianus | ? | - |
? | |
additional information | arginine-dependent ATP-diphosphate exchange | Saccharomyces pastorianus 836 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 74000, SDS-PAGE | Saccharomyces pastorianus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
24 h, stable, without glycerol | Saccharomyces pastorianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
aminoacylation | Saccharomyces pastorianus |