Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
argS MA5002 | mutant argS MA5002 differs from the wild-type ArgRS structural gene by one mutation, a substitution of an Arg by a Ser at position 134. It exhibits a 4times to 6times as low activity and a 5times as high Km value for ATP as the wild-type enzyme in aminoacylation and ATP-diphosphate exchange, Km values for Arg and tRNAArg remain unaltered | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
tRNAArg | aminoacylation, native enzyme | Escherichia coli | |
0.0042 | - |
tRNAArg | aminoacylation, mutant enzyme | Escherichia coli | |
0.009 | - |
Arg | aminoacylation, native enzyme | Escherichia coli | |
0.011 | - |
Arg | aminoacylation and ATP-diphosphate exchange, mutant enzyme | Escherichia coli | |
0.11 | - |
Arg | ATP-diphosphate exchange | Escherichia coli | |
0.15 | - |
Arg | ATP-diphosphate exchange, native enzyme | Escherichia coli | |
0.7 | - |
Arg | aminoacylation, native enzyme | Escherichia coli | |
2 | - |
ATP | ATP-diphosphate exchange, native enzyme | Escherichia coli | |
4.3 | - |
ATP | aminoacylation, mutant enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
mutant argS MA5002 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Escherichia coli | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
additional information | arginine-dependent ATP-diphosphate exchange | Escherichia coli | ? | - |
? |