Literature summary for 6.1.1.18 extracted from

van Rooyen, J.M.; Hakimi, M.A.; Belrhali, H.
Recombinant expression, purification, and crystallization of the glutaminyl-tRNA synthetase from Toxoplasma gondii (2015), Protein Expr. Purif., 110, 115-121 .

Application

Application	Comment	Organism
drug development	the essential nature of the enzymes makes it an attractive targets for designing of drugs against important pathogenic protozoans like Toxoplasma	Toxoplasma gondii

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His6-tagged wild-type full-length enzyme and truncated mutant enzymes in Escherichia coli strain BL21(DE3), optimization of expression conditions	Toxoplasma gondii

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged N-terminal and C-terminal domain fragments, sitting drop vapour diffusion method, mixing of 20 mg/ml protein in 25 mM Tris pH 7.5, 150 mM KCl, and 10 mM MgCl2, with 2 M ammonium sulfate, 5% isopropanol for the N-terminal fragment and 2 M ammonium sulfate for the C-terminal fragment, X-ray diffraction structure determination and analysis. No crystallization conditions can be identified for the full-length protein	Toxoplasma gondii

Crystallization (Comment)

Organism

purified recombinant His-tagged N-terminal and C-terminal domain fragments, sitting drop vapour diffusion method, mixing of 20 mg/ml protein in 25 mM Tris pH 7.5, 150 mM KCl, and 10 mM MgCl2, with 2 M ammonium sulfate, 5% isopropanol for the N-terminal fragment and 2 M ammonium sulfate for the C-terminal fragment, X-ray diffraction structure determination and analysis. No crystallization conditions can be identified for the full-length protein

Toxoplasma gondii

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a C-terminal catalytic domain (QRS-C) of the QRS gene, a region corresponding to residues 255-805 of the annotated full-length gene, and of an N-terminal domain contruct	Toxoplasma gondii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-glutamine + tRNAGln	Toxoplasma gondii	-	AMP + diphosphate + L-glutaminyl-tRNAGln	-	?
ATP + L-glutamine + tRNAGln	Toxoplasma gondii ATCC 50611 / Me49	-	AMP + diphosphate + L-glutaminyl-tRNAGln	-	?

Organism

Organism	UniProt	Comment	Textmining
Toxoplasma gondii	A0A125YXL3	-	-
Toxoplasma gondii ATCC 50611 / Me49	A0A125YXL3	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged wild-type full-length enzyme and truncated mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration, optimization of purification conditions	Toxoplasma gondii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-glutamine + tRNAGln	-	Toxoplasma gondii	AMP + diphosphate + L-glutaminyl-tRNAGln	-	?
ATP + L-glutamine + tRNAGln	-	Toxoplasma gondii ATCC 50611 / Me49	AMP + diphosphate + L-glutaminyl-tRNAGln	-	?

Synonyms

Synonyms	Comment	Organism
Glutaminyl-tRNA synthetase	-	Toxoplasma gondii
QRS	-	Toxoplasma gondii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Toxoplasma gondii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Toxoplasma gondii

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Toxoplasma gondii