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Literature summary for 6.1.1.18 extracted from
- How glutaminyl-tRNA synthetase selects glutamine (1998), Structure, 6, 439-449.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| cocrystallization of the purified enzyme with tRNAGln and inhibitor QSI, X-ray diffraction structure determination at 2.4 A resolution and analysis | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-O-[N-(L-glutaminyl)sulfamoyl] adenosine | i.e. QSI, glutaminyl-adenylate analogue, competitive to glutamine | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamine + tRNAGln | Escherichia coli | - |
AMP + diphosphate + L-glutaminyl-tRNAGln | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P00962 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-glutamine + tRNAGln = AMP + diphosphate + L-glutaminyl-tRNAGln | aminoacylation reaction mechanism, substrate binding sites, Tyr211 and a water molecule are responsible for recognition of both hydrogen atoms of the nitrogen of glutamine sidechain, which is esstial for substrate recognition | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamine + tRNAGln | - |
Escherichia coli | AMP + diphosphate + L-glutaminyl-tRNAGln | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlnRS | - |
Escherichia coli |
| Glutaminyl-tRNA synthetase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
5'-O-[N-(L-glutaminyl)sulfamoyl] adenosine | pH 7.0, 37°C | Escherichia coli | |
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Release 2023.1 (January 2023)
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