Protein Variants | Comment | Organism |
---|---|---|
F90L | site-directed mutagenesis, active site mutant, 5fold improved glutamic acid recognition in vitro, in vivo the mutant shows a 40% reduced growth rate, partial complementation of an enzyme-deficient strain | Escherichia coli |
additional information | construction of a truncated enzyme form, partial complementation of an enzyme-deficient strain, reduced growth rate in vivo | Escherichia coli |
Y240E | site-directed mutagenesis, active site mutant, 5fold improved glutamic acid recognition in vitro, partial complementation of an enzyme-deficient strain | Escherichia coli |
Y240G | site-directed mutagenesis, active site mutant, 3fold improved glutamic acid recognition in vitro, partial complementation of an enzyme-deficient strain | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Glutamic acid | competitive inhibition of the wild-type and mutant enzymes | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
L-glutamine | mutant F90L, pH 7.2, 37°C | Escherichia coli | |
0.06 | - |
L-glutamine | truncated mutant, pH 7.2, 37°C | Escherichia coli | |
0.07 | - |
L-glutamine | mutant Y240E, pH 7.2, 37°C | Escherichia coli | |
0.12 | - |
L-glutamine | mutant Y240G, pH 7.2, 37°C | Escherichia coli | |
0.19 | - |
L-glutamine | wild-type enzyme, pH 7.2, 37°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + tRNAGln | Escherichia coli | - |
AMP + diphosphate + L-glutaminyl-tRNAGln | - |
? | |
additional information | Escherichia coli | phylogenetic analysis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
purified recombinant wild-type and mutant enzymes | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-glutamine + tRNAGln = AMP + diphosphate + L-glutaminyl-tRNAGln | active site residues are Tyr240 and Phe90 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + tRNAGln | - |
Escherichia coli | AMP + diphosphate + L-glutaminyl-tRNAGln | - |
? | |
additional information | phylogenetic analysis | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlnRS | - |
Escherichia coli |
Glutaminyl-tRNA synthetase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
L-glutamine | mutant Y240E, pH 7.2, 37°C | Escherichia coli | |
2 | - |
L-glutamine | mutant F90L, pH 7.2, 37°C | Escherichia coli | |
3.4 | - |
L-glutamine | mutant Y240G, pH 7.2, 37°C | Escherichia coli | |
4.7 | - |
L-glutamine | wild-type enzyme and truncated mutant, pH 7.2, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
L-Glutamic acid | truncated mutant, pH 7.2, 37°C | Escherichia coli | |
21 | - |
L-Glutamic acid | mutant Y240E, pH 7.2, 37°C | Escherichia coli | |
29 | - |
L-Glutamic acid | mutant F90L, pH 7.2, 37°C | Escherichia coli | |
47 | - |
L-Glutamic acid | mutant Y240G, pH 7.2, 37°C | Escherichia coli | |
96 | - |
L-Glutamic acid | wild-type enzyme, pH 7.2, 37°C | Escherichia coli |