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Literature summary for 6.1.1.18 extracted from

  • Liu, J.; Ibba, M.; Hong, K.W.; Soll, D.
    The terminal adenosine of tRNA(Gln) mediates tRNA-dependent amino acid recognition by glutaminyl-tRNA synthetase (1998), Biochemistry, 37, 9836-9842.
    View publication on PubMed
Search for more literature for 6.1.1.18

Protein Variants

Protein Variants Comment Organism
D235A saturation mutagenesis, only little complementation of glnS-deficient strain Escherichia coli
D66E saturation mutagenesis, 18fold increased Km for glutamine, decreased turnover Escherichia coli
D66F saturation mutagenesis, highly increased Km for glutamine, 1200fold decrease in activity Escherichia coli
D66G saturation mutagenesis, only little complementation of glnS-deficient strain Escherichia coli
D66H saturation mutagenesis, only little complementation of glnS-deficient strain Escherichia coli
D66R saturation mutagenesis, only little complementation of glnS-deficient strain Escherichia coli
F233D saturation mutagenesis, highly increased Km for glutamine, 3700fold decrease in activity Escherichia coli
F233L saturation mutagenesis, 19fold increased Km for glutamine, decreased turnover Escherichia coli
F233Y saturation mutagenesis, increased Km for glutamine, increased turnover Escherichia coli
Y211F saturation mutagenesis, 60fold increased Km for glutamine, decreased turnover Escherichia coli
Y211F/F233Y saturation mutagenesis, increased Km for glutamine, about 6fold decreased activity Escherichia coli
Y211G saturation mutagenesis, only little complementation of glnS-deficient strain Escherichia coli
Y211L saturation mutagenesis, unaffected Km for glutamine, decreased turnover Escherichia coli
Y211S saturation mutagenesis, 1700fold decrease in activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 ATP wild-type enzyme, pH 7.2, 37°C Escherichia coli
0.114
-
 L-glutamine wild-type enzyme, pH 7.2, 37°C Escherichia coli
0.118
-
 L-glutamine mutant Y211L, pH 7.2, 37°C Escherichia coli
0.13
-
 ATP mutant D66E, pH 7.2, 37°C Escherichia coli
0.25
-
 ATP mutant Y211F/F233Y, pH 7.2, 37°C Escherichia coli
0.32
-
 ATP mutant Y211L, pH 7.2, 37°C Escherichia coli
0.42
-
 ATP mutant F233Y, pH 7.2, 37°C Escherichia coli
0.53
-
 L-glutamine mutant F233Y, pH 7.2, 37°C Escherichia coli
0.54
-
 ATP mutant F233D, pH 7.2, 37°C Escherichia coli
0.58
-
 L-glutamine mutant Y211F/F233Y, pH 7.2, 37°C Escherichia coli
0.59
-
 ATP mutant D66F, pH 7.2, 37°C Escherichia coli
0.735
-
 ATP mutant Y211S, pH 7.2, 37°C Escherichia coli
0.74
-
 ATP mutant Y211F, pH 7.2, 37°C Escherichia coli
0.75
-
 ATP mutant F233L, pH 7.2, 37°C Escherichia coli
0.76
-
 L-glutamine mutant D66F, pH 7.2, 37°C Escherichia coli
2.02
-
 L-glutamine mutant D66E, pH 7.2, 37°C Escherichia coli
2.21
-
 L-glutamine mutant F233L, pH 7.2, 37°C Escherichia coli
6.05
-
 L-glutamine mutant Y211S, pH 7.2, 37°C Escherichia coli
7.05
-
 L-glutamine mutant Y211F, pH 7.2, 37°C Escherichia coli
7.76
-
 L-glutamine mutant F233D, pH 7.2, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamine + tRNAGln Escherichia coli
-
 AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 strains BT3213 and KL2576
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamine + tRNAGln
-
 Escherichia coli AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?
ATP + L-glutamine + tRNAGln specific tRNA-dependent amino acid recognition involves Asp66, Tyr211, and Phe233, which interact with A76 of tRNAGln and glutamine Escherichia coli AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?

Synonyms

Synonyms Comment Organism
Glutaminyl-tRNA synthetase
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 ATP mutant D66E, pH 7.2, 37°C Escherichia coli
0.014
-
 L-glutamine mutant D66F, pH 7.2, 37°C Escherichia coli
0.016
-
 ATP mutant D66F, pH 7.2, 37°C Escherichia coli
0.042
-
 ATP mutant F233D, pH 7.2, 37°C Escherichia coli
0.05
-
 L-glutamine mutant F233D, pH 7.2, 37°C Escherichia coli
0.082
-
 L-glutamine mutant Y211S, pH 7.2, 37°C Escherichia coli
0.14
-
 ATP mutant Y211S, pH 7.2, 37°C Escherichia coli
0.32
-
 ATP mutant Y211L, pH 7.2, 37°C Escherichia coli
0.4
-
 L-glutamine mutant Y211L, pH 7.2, 37°C Escherichia coli
0.46
-
 ATP mutant Y211F/F233Y, pH 7.2, 37°C Escherichia coli
0.55
-
 L-glutamine mutant Y211F/F233Y, pH 7.2, 37°C Escherichia coli
0.7
-
 L-glutamine mutant D66E, pH 7.2, 37°C Escherichia coli
1.48
-
 L-glutamine mutant Y211F, pH 7.2, 37°C Escherichia coli
1.51
-
 ATP mutant Y211F, pH 7.2, 37°C Escherichia coli
1.62
-
 L-glutamine mutant F233L, pH 7.2, 37°C Escherichia coli
2.47
-
 ATP mutant F233Y, pH 7.2, 37°C Escherichia coli
2.62
-
 L-glutamine wild-type enzyme, pH 7.2, 37°C Escherichia coli
2.75
-
 ATP mutant F233L, pH 7.2, 37°C Escherichia coli
2.8
-
 ATP wild-type enzyme, pH 7.2, 37°C Escherichia coli
2.94
-
 L-glutamine mutant F233L, pH 7.2, 37°C Escherichia coli
2.94
-
 ATP mutant F233Y, pH 7.2, 37°C Escherichia coli
2.94
-
 ATP mutant Y211F, pH 7.2, 37°C Escherichia coli
3 6 L-glutamine mutant F233Y, pH 7.2, 37°C Escherichia coli
3 6 L-glutamine wild-type enzyme, pH 7.2, 37°C Escherichia coli
3.02
-
 L-glutamine mutant F233Y, pH 7.2, 37°C Escherichia coli
6.08
-
 L-glutamine mutant Y211F, pH 7.2, 37°C Escherichia coli
6.27
-
 ATP mutant D66E, pH 7.2, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli