Protein Variants | Comment | Organism |
---|---|---|
additional information | temperature-sensitive mutant enzyme, no change in affinity for glutamine | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
temperature-resistant strain KL301 and temperature-sensitive mutant | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + tRNAGln | - |
Escherichia coli | AMP + diphosphate + L-glutaminyl-tRNAGln | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
strain KL301 enzyme is stable, temperature-sensitive mutant enzyme loses about 70% of its activity | Escherichia coli |