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Literature summary for 6.1.1.17 extracted from
- An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli (2003), Nucleic Acids Res., 31, 6700-6709.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants | Saccharolobus solfataricus |
| cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants | Methanocaldococcus jannaschii |
| cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants | Archaeoglobus fulgidus |
| cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants | Pyrococcus horikoshii |
| cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants | Methanosarcina mazei |
| cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants | Aeropyrum pernix |
| cloning and overexpression in Escherichia coli, coexpression of recombinant tRNA mutant variants | Methanococcus thermoautotrophicum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 | Saccharolobus solfataricus |
| additional information | design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 | Methanocaldococcus jannaschii |
| additional information | design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 | Archaeoglobus fulgidus |
| additional information | design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 | Pyrococcus horikoshii |
| additional information | design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 | Methanosarcina mazei |
| additional information | design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 | Aeropyrum pernix |
| additional information | design of orthogonal tRNAs with sequences derived from archaeal tRNAs, 3 forms: 1. AQ(GU), i.e. archaeal glutaminyl with GU base pair at position 10-26, 2. AE(GU), i.e. archaeal glutamyl with GU base pair at position 10-28, 3. AE(GC), i.e. archaeal glutamyl with GC base pair at position 10-28 | Methanococcus thermoautotrophicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + tRNAGlu | Saccharolobus solfataricus | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |  |
| ATP + L-glutamate + tRNAGlu | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | Archaeoglobus fulgidus | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | Pyrococcus horikoshii | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | Methanosarcina mazei | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | Aeropyrum pernix | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | Methanococcus thermoautotrophicum | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeropyrum pernix | - |
- |
- |
| Archaeoglobus fulgidus | - |
- |
- |
| Methanocaldococcus jannaschii | - |
- |
- |
| Methanococcus thermoautotrophicum | - |
- |
- |
| Methanosarcina mazei | - |
- |
- |
| Pyrococcus horikoshii | - |
- |
- |
| Saccharolobus solfataricus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + tRNAGlu | - |
Saccharolobus solfataricus | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | - |
Archaeoglobus fulgidus | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | - |
Pyrococcus horikoshii | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | - |
Methanosarcina mazei | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | - |
Aeropyrum pernix | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | - |
Methanococcus thermoautotrophicum | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | wild-type tRNAGlu and tRNA AE(GU) | Saccharolobus solfataricus | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | wild-type tRNAGlu and tRNA AE(GU) | Methanocaldococcus jannaschii | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | wild-type tRNAGlu and tRNA AE(GU) | Archaeoglobus fulgidus | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | wild-type tRNAGlu and tRNA AE(GU) | Pyrococcus horikoshii | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | wild-type tRNAGlu and tRNA AE(GU) | Methanosarcina mazei | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | wild-type tRNAGlu and tRNA AE(GU) | Aeropyrum pernix | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| ATP + L-glutamate + tRNAGlu | wild-type tRNAGlu and tRNA AE(GU) | Methanococcus thermoautotrophicum | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
| additional information | no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) | Saccharolobus solfataricus | ? | - |
? | |
| additional information | no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) | Methanocaldococcus jannaschii | ? | - |
? | |
| additional information | no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) | Archaeoglobus fulgidus | ? | - |
? | |
| additional information | no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) | Pyrococcus horikoshii | ? | - |
? | |
| additional information | no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) | Methanosarcina mazei | ? | - |
? | |
| additional information | no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) | Aeropyrum pernix | ? | - |
? | |
| additional information | no recognition of recombinant tRNA mutants AQ(GU) and AQ(GC) | Methanococcus thermoautotrophicum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GluRS | - |
Methanocaldococcus jannaschii |
| Glutamate--tRNA ligase | - |
Methanocaldococcus jannaschii |
| Glutamate-tRNA synthetase | - |
Methanocaldococcus jannaschii |
| Glutamic acid translase | - |
Methanocaldococcus jannaschii |
| Glutamic acid tRNA ligase | - |
Methanocaldococcus jannaschii |
| Glutamyl tRNA synthetase | - |
Methanocaldococcus jannaschii |
| Glutamyl-transfer ribonucleate synthetase | - |
Methanocaldococcus jannaschii |
| Glutamyl-transfer ribonucleic acid synthetase | - |
Methanocaldococcus jannaschii |
| Glutamyl-transfer RNA synthetase | - |
Methanocaldococcus jannaschii |
| Glutamyl-tRNA synthetase | - |
Saccharolobus solfataricus |
| Glutamyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
| Glutamyl-tRNA synthetase | - |
Archaeoglobus fulgidus |
| Glutamyl-tRNA synthetase | - |
Pyrococcus horikoshii |
| Glutamyl-tRNA synthetase | - |
Methanosarcina mazei |
| Glutamyl-tRNA synthetase | - |
Aeropyrum pernix |
| Glutamyl-tRNA synthetase | - |
Methanococcus thermoautotrophicum |
| P85 | - |
Methanocaldococcus jannaschii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharolobus solfataricus | |
| ATP | - |
Methanocaldococcus jannaschii | |
| ATP | - |
Archaeoglobus fulgidus | |
| ATP | - |
Pyrococcus horikoshii | |
| ATP | - |
Methanosarcina mazei | |
| ATP | - |
Aeropyrum pernix | |
| ATP | - |
Methanococcus thermoautotrophicum | |
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