Literature summary for 6.1.1.17 extracted from

Sekine, S.; Nureki, O.; Shimada, A.; Vassylyev, D.G.; Yokoyama, S.
Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase (2001), Nat. Struct. Biol., 8, 203-206.

Search for more literature for 6.1.1.17

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme in complex with tRNAGlu, hanging-drop vapour diffusion method, precipitant solution contains 37 mM Na-MOPS, pH 6.7, 22% PEG 1500, 37 mM ammonium sulfate, 1% 2-methyl-2,4-pentanediol, 10 mM MgCl2, 5 mM 2-mercaptoethanol, X-ray diffraction structure determination at 2.4 A resolution, and analysis	Thermus thermophilus

Protein Variants

Protein Variants	Comment	Organism
R358Q	site-directed mutagenesis, exchange of the Arg residue results in a mutant that no longer discriminates between tRNAGlu and tRNAGln anticodons YUC and YUG, respectively	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0047	-	wild type tRNAGlu	recombinant wild-type enzyme, 65°C	Thermus thermophilus
0.043	-	tRNAGlu mutant C36G	recombinant wild-type enzyme, 65°C	Thermus thermophilus
0.055	-	tRNAGlu mutant C36G	recombinant mutant R358Q, 65°C	Thermus thermophilus
0.085	-	wild type tRNAGlu	recombinant mutant R358Q, 65°C	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-glutamate + tRNAGlu	Thermus thermophilus	-	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	P27000	discriminating enzyme form, purified recombinant enzyme from overexpressing Escherichia coli strain JM109(DE3)	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-glutamate + tRNAGlu	-	Thermus thermophilus	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?
ATP + L-glutamate + tRNAGlu mutant C36G	mutant R358Q, low activity with the wild-type enzyme	Thermus thermophilus	AMP + diphosphate + L-glutamyl-tRNAGlu mutant C36G	-	?
ATP + L-glutamate + tRNAGlu wild-type	enzyme is specific for tRNAGlu	Thermus thermophilus	AMP + diphosphate + L-glutamyl-tRNAGlu wild-type	-	?
ATP + L-glutamate + wild type tRNAGlu	-	Thermus thermophilus	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?

Synonyms

Synonyms	Comment	Organism
GluRS	-	Thermus thermophilus
Glutamyl-tRNA synthetase	-	Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	assay at	Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.18	-	tRNAGlu mutant C36G	recombinant wild-type enzyme, 65°C	Thermus thermophilus
1.4	-	tRNAGlu mutant C36G	recombinant mutant enzyme R358Q, 65°C	Thermus thermophilus
1.5	-	wild type tRNAGlu	recombinant mutant enzyme R358Q, 65°C	Thermus thermophilus
2.1	-	wild type tRNAGlu	recombinant wild-type enzyme, 65°C	Thermus thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Thermus thermophilus

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Release 2023.1 (January 2023)