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Literature summary for 6.1.1.17 extracted from

  • Nunez, H.; Lefimil, C.; Min, B.; Soll, D.; Orellana, O.
    In vivo formation of glutamyl-tRNA(Gln) in Escherichia coli by heterologous glutamyl-tRNA synthetases (2004), FEBS Lett., 557, 133-135.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acidithiobacillus ferrooxidans
gene gltX mutant, mutant enzyme expression in Escherichia coli temperature-sensitive mutant strain Bacillus subtilis
gene gltX1 and gltX2, expression in Escherichia coli temperature-sensitive mutant strain Acidithiobacillus ferrooxidans
gene gltX2, expression in Escherichia coli temperature-sensitive mutant strain Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
Q373R substrate specificity restricted to tRNAGlu compared to the wild-type which also accepts tRNAGln Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + tRNAGln Helicobacter pylori enzyme expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGln
-
?
ATP + L-glutamate + tRNAGln Acidithiobacillus ferrooxidans isozyme 2 expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGln
-
?
ATP + L-glutamate + tRNAGlu Helicobacter pylori
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
ATP + L-glutamate + tRNAGlu Acidithiobacillus ferrooxidans isozyme 1 expressed in an Escherichia coli mutant strain, tRNAGlu from Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
ATP + L-glutamate + tRNAGlu Bacillus subtilis recombinant mutant Q373R expressed in Escherichia coli mutant strain, tRNAGlu from Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
additional information Bacillus subtilis no charging of Escherichia coli tRNAGln by enzyme mutant Q373R ?
-
?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans
-
-
-
Acidithiobacillus ferrooxidans
-
strain ATCC 23270, isoform 1 and 2, genes gltX1 and gltX2
-
Bacillus subtilis
-
-
-
Helicobacter pylori
-
isozyme 2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Glu + tRNAGln
-
Acidithiobacillus ferrooxidans AMP + diphosphate + L-glutamyl-tRNAGln
-
?
ATP + L-glutamate + tRNAGln enzyme expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli Helicobacter pylori AMP + diphosphate + L-glutamyl-tRNAGln
-
?
ATP + L-glutamate + tRNAGln isozyme 2 expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli Acidithiobacillus ferrooxidans AMP + diphosphate + L-glutamyl-tRNAGln
-
?
ATP + L-glutamate + tRNAGlu
-
Helicobacter pylori AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
ATP + L-glutamate + tRNAGlu isozyme 1 expressed in an Escherichia coli mutant strain, tRNAGlu from Escherichia coli Acidithiobacillus ferrooxidans AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
ATP + L-glutamate + tRNAGlu recombinant mutant Q373R expressed in Escherichia coli mutant strain, tRNAGlu from Escherichia coli Bacillus subtilis AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
additional information no charging of Escherichia coli tRNAGln by enzyme mutant Q373R Bacillus subtilis ?
-
?

Synonyms

Synonyms Comment Organism
GluRS
-
Acidithiobacillus ferrooxidans
GluRS
-
Bacillus subtilis
GluRS
-
Helicobacter pylori
Glutamyl-tRNA synthetase
-
Acidithiobacillus ferrooxidans
Glutamyl-tRNA synthetase
-
Bacillus subtilis
Glutamyl-tRNA synthetase
-
Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
ATP
-
Acidithiobacillus ferrooxidans
ATP
-
Bacillus subtilis
ATP
-
Helicobacter pylori