Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Acidithiobacillus ferrooxidans |
gene gltX mutant, mutant enzyme expression in Escherichia coli temperature-sensitive mutant strain | Bacillus subtilis |
gene gltX1 and gltX2, expression in Escherichia coli temperature-sensitive mutant strain | Acidithiobacillus ferrooxidans |
gene gltX2, expression in Escherichia coli temperature-sensitive mutant strain | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
Q373R | substrate specificity restricted to tRNAGlu compared to the wild-type which also accepts tRNAGln | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGln | Helicobacter pylori | enzyme expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli | AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGln | Acidithiobacillus ferrooxidans | isozyme 2 expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli | AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGlu | Helicobacter pylori | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | Acidithiobacillus ferrooxidans | isozyme 1 expressed in an Escherichia coli mutant strain, tRNAGlu from Escherichia coli | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | Bacillus subtilis | recombinant mutant Q373R expressed in Escherichia coli mutant strain, tRNAGlu from Escherichia coli | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
additional information | Bacillus subtilis | no charging of Escherichia coli tRNAGln by enzyme mutant Q373R | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | - |
- |
- |
Acidithiobacillus ferrooxidans | - |
strain ATCC 23270, isoform 1 and 2, genes gltX1 and gltX2 | - |
Bacillus subtilis | - |
- |
- |
Helicobacter pylori | - |
isozyme 2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Glu + tRNAGln | - |
Acidithiobacillus ferrooxidans | AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGln | enzyme expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli | Helicobacter pylori | AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGln | isozyme 2 expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli | Acidithiobacillus ferrooxidans | AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGlu | - |
Helicobacter pylori | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | isozyme 1 expressed in an Escherichia coli mutant strain, tRNAGlu from Escherichia coli | Acidithiobacillus ferrooxidans | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | recombinant mutant Q373R expressed in Escherichia coli mutant strain, tRNAGlu from Escherichia coli | Bacillus subtilis | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
additional information | no charging of Escherichia coli tRNAGln by enzyme mutant Q373R | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GluRS | - |
Acidithiobacillus ferrooxidans |
GluRS | - |
Bacillus subtilis |
GluRS | - |
Helicobacter pylori |
Glutamyl-tRNA synthetase | - |
Acidithiobacillus ferrooxidans |
Glutamyl-tRNA synthetase | - |
Bacillus subtilis |
Glutamyl-tRNA synthetase | - |
Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Acidithiobacillus ferrooxidans | |
ATP | - |
Bacillus subtilis | |
ATP | - |
Helicobacter pylori |