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Literature summary for 6.1.1.17 extracted from

  • Sekine, S.I.; Nureki, O.; Tateno, M.; Yokoyama, S.
    The identity determinants required for the discrimination between tRNAGlu and tRNAAsp by glutamyl-tRNA synthetase from Escherichia coli (1999), Eur. J. Biochem., 261, 354-360.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km for diverse modified forms of tRNAAsp and tRNAGlu Escherichia coli
0.056
-
tRNAGlu wild-type tRNA substrate, pH 7.2, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + tRNAGlu Escherichia coli
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + tRNAGlu
-
Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
additional information the identity of tRNAGlu is determined by the bases set U34,U35, C36, A37, G1.C72, U2.A71, U11.A24, U13.G22..A46, and DELTA47, if this set is transplanted to tRNAAsp in addition to C4.G69 and C12.G23..C9, the tRNAAsp is a substrate for the enzyme, while tRNAGlu modification at the bases of the determinant set results in reduced activity Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
GluRS
-
Escherichia coli
Glutamyl-tRNA synthetase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information activity with diverse modified forms of tRNAAsp and tRNAGlu Escherichia coli
3
-
tRNAGlu wild-type tRNA substrate, pH 7.2, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli