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Literature summary for 6.1.1.16 extracted from

  • Ravishankar, S.; Ambady, A.; Swetha, R.G.; Anbarasu, A.; Ramaiah, S.; Sambandamurthy, V.K.
    Essentiality assessment of cysteinyl and lysyl-tRNA synthetases of Mycobacterium smegmatis (2016), PLoS ONE, 11, e0147188 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.16

Application

Application Comment Organism
drug development aminoacyl-tRNA synthetases are attractive targets for the discovery of antibacterial agents Mycolicibacterium smegmatis

Cloned(Commentary)

Cloned (Comment) Organism
gene cysS, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in conditional expression strains of Mycobacterium smegmatis strain mc2155 from pAZI9452 vector Mycolicibacterium smegmatis

Protein Variants

Protein Variants Comment Organism
additional information generation of conditional expression strains, conditional expression plasmids are electroporated into Mycobacterium smegmatis strain mc2155, analysis of inducer dependency of conditional expression strains, overview Mycolicibacterium smegmatis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane transmembrane segment analysis of lysyl-tRNA synthetase Mycolicibacterium smegmatis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-cysteine + tRNACys Mycolicibacterium smegmatis
-
 AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
ATP + L-cysteine + tRNACys Mycobacterium leprae
-
 AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
ATP + L-cysteine + tRNACys Mycolicibacterium smegmatis mc2155 / ATCC 700084
-
 AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium leprae
-
-
-
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium smegmatis A0R578 gene cysS
-
Mycolicibacterium smegmatis mc2155 / ATCC 700084 A0R578 gene cysS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNACys
-
 Mycolicibacterium smegmatis AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
ATP + L-cysteine + tRNACys
-
 Mycobacterium leprae AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
ATP + L-cysteine + tRNACys
-
 Mycolicibacterium smegmatis mc2155 / ATCC 700084 AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Synonyms

Synonyms Comment Organism
CysRS
-
 Mycolicibacterium smegmatis
CysRS
-
 Mycobacterium leprae
cysS
-
 Mycolicibacterium smegmatis
cysteinyl tRNA synthetase
-
 Mycolicibacterium smegmatis
cysteinyl tRNA synthetase
-
 Mycobacterium leprae
Cysteinyl-tRNA synthetase
-
 Mycolicibacterium smegmatis
MSMEG_6074
-
 Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mycolicibacterium smegmatis
ATP
-
 Mycobacterium leprae

General Information

General Information Comment Organism
evolution multiple genes encode for aminoacyl-tRNA synthetases from Mycobacterium smegmatis, e.g. glutamyl (GluRS), cysteinyl (CysRS), prolyl (ProRS) and lysyl (LysRS) tRNA synthetases Mycolicibacterium smegmatis
physiological function Essentiality of lysyl and cysteinyl-tRNA synthetases of Mycobacterium smegmatis Mycolicibacterium smegmatis
physiological function the enzyme is essential for growth Mycolicibacterium smegmatis