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Literature summary for 6.1.1.16 extracted from

  • Ghosh, A.; Sakaguchi, R.; Liu, C.; Vishveshwara, S.; Hou, Y.M.
    Allosteric communication in cysteinyl tRNA synthetase: a network of direct and indirect readout (2011), J. Biol. Chem., 286, 37721-37731.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
E354Q the mutant has decreased kcat and Km values leading to an overall decrease in kcat/Km by 6.8fold relative to the wild type enzyme Escherichia coli
E354Q/R427A the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 22fold relative to the wild type enzyme Escherichia coli
H297A the mutant has a decreased kcat and Km values leading to an overall decrease in kcat/Km by 3.4fold relative to the wild type enzyme Escherichia coli
H404A/R42A the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 209fold relative to the wild type enzyme Escherichia coli
H40A the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 204fold relative to the wild type enzyme Escherichia coli
M294A the mutant has decreased kcat and Km values leading to an overall decrease in kcat/Km by 3.7fold relative to the wild type enzyme Escherichia coli
M294A/H297A the mutant has a decreased kcat and Km values leading to an overall decrease in kcat/Km by 8.1fold relative to the wild type enzyme Escherichia coli
M294A/R427A the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 370fold relative to the wild type enzyme Escherichia coli
R427A the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 291fold relative to the wild type enzyme Escherichia coli
R42A the mutant has a decreased kcat and an increased Km leading to an overall decrease in kcat/Km by 1.5fold relative to the wild type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00047
-
 L-cysteine mutant enzyme M294A, pH and temperature not specified in the publication Escherichia coli
0.00052
-
 L-cysteine mutant enzyme E354Q, pH and temperature not specified in the publication Escherichia coli
0.00055
-
 L-cysteine mutant enzyme H297A, pH and temperature not specified in the publication Escherichia coli
0.00066
-
 L-cysteine mutant enzyme M294A/H297A, pH and temperature not specified in the publication Escherichia coli
0.0012
-
 L-cysteine wild type enzyme, pH and temperature not specified in the publication Escherichia coli
0.0018
-
 L-cysteine mutant enzyme R42A, pH and temperature not specified in the publication Escherichia coli
0.002
-
 L-cysteine mutant enzyme H40A, pH and temperature not specified in the publication Escherichia coli
0.0075
-
 L-cysteine mutant enzyme R427A, pH and temperature not specified in the publication Escherichia coli
0.0076
-
 L-cysteine mutant enzyme M294A/R427A, pH and temperature not specified in the publication Escherichia coli
0.01
-
 L-cysteine mutant enzyme E354Q/R427A, pH and temperature not specified in the publication Escherichia coli
2.4
-
 L-cysteine mutant enzyme H40A/R42A, pH and temperature not specified in the publication Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P21888
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon resin column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNACys
-
 Escherichia coli AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Synonyms

Synonyms Comment Organism
CysRS
-
 Escherichia coli
cysteinyl tRNA synthetase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 L-cysteine mutant enzyme H40A/R42A, pH and temperature not specified in the publication Escherichia coli
0.0093
-
 L-cysteine mutant enzyme H40A, pH and temperature not specified in the publication Escherichia coli
0.02
-
 L-cysteine mutant enzyme M294A/R427A, pH and temperature not specified in the publication Escherichia coli
0.027
-
 L-cysteine mutant enzyme R427A, pH and temperature not specified in the publication Escherichia coli
0.082
-
 L-cysteine mutant enzyme E354Q, pH and temperature not specified in the publication Escherichia coli
0.088
-
 L-cysteine mutant enzyme M294A/H297A, pH and temperature not specified in the publication Escherichia coli
0.14
-
 L-cysteine mutant enzyme M294A, pH and temperature not specified in the publication Escherichia coli
0.16
-
 L-cysteine mutant enzyme H297A, pH and temperature not specified in the publication Escherichia coli
0.49
-
 L-cysteine mutant enzyme E354Q/R427A, pH and temperature not specified in the publication Escherichia coli
1.1
-
 L-cysteine mutant enzyme R42A, pH and temperature not specified in the publication Escherichia coli
1.2
-
 L-cysteine wild type enzyme, pH and temperature not specified in the publication Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3
-
 L-cysteine mutant enzyme M294A/R427A, pH and temperature not specified in the publication Escherichia coli
3.8
-
 L-cysteine mutant enzyme R427A, pH and temperature not specified in the publication Escherichia coli
5.3
-
 L-cysteine mutant enzyme H40A/R42A, pH and temperature not specified in the publication Escherichia coli
5.4
-
 L-cysteine mutant enzyme H40A, pH and temperature not specified in the publication Escherichia coli
50
-
 L-cysteine mutant enzyme E354Q/R427A, pH and temperature not specified in the publication Escherichia coli
140
-
 L-cysteine mutant enzyme M294A/H297A, pH and temperature not specified in the publication Escherichia coli
160
-
 L-cysteine mutant enzyme E354Q, pH and temperature not specified in the publication Escherichia coli
300
-
 L-cysteine mutant enzyme M294A, pH and temperature not specified in the publication Escherichia coli
320
-
 L-cysteine mutant enzyme H297A, pH and temperature not specified in the publication Escherichia coli
750
-
 L-cysteine mutant enzyme R42A, pH and temperature not specified in the publication Escherichia coli
1000
-
 L-cysteine wild type enzyme, pH and temperature not specified in the publication Escherichia coli