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Literature summary for 6.1.1.16 extracted from
- Shape-selective RNA recognition by cysteinyl-tRNA synthetase (2004), Nat. Struct. Mol. Biol., 11, 1134-1141.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, crystals of enzyme bound to tRNACys at a resolution of 2.3 A | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
tRNACys | mutant enzyme E354A, wild-type tRNACys(G15-G48) | Escherichia coli |  |
| 0.00058 | - |
tRNACys | mutant enzyme E354Q, wild-type tRNACys(G15-G48) | Escherichia coli | |
| 0.00116 | - |
tRNACys | wild-type enzyme, wild-type tRNACys(G15-G48) | Escherichia coli | |
| 0.0088 | - |
tRNACys | mutant enzyme N351A, wild-type tRNACys(G15-G48) | Escherichia coli | |
| 0.0177 | - |
tRNACys | mutant enzyme N351D, mutant tRNACys(G15-C48) | Escherichia coli | |
| 0.0179 | - |
tRNACys | mutant enzyme E354Q, wild-type tRNACys(G15-G48) | Escherichia coli | |
| 0.0185 | - |
tRNACys | mutant enzyme N351D, wild-type tRNACys(G15-G48) | Escherichia coli | |
| 0.0332 | - |
tRNACys | mutant enzyme N351A, mutant tRNACys(G15-C48) | Escherichia coli | |
| 0.0358 | - |
tRNACys | wild-type enzyme, mutant tRNACys(G15-C48) | Escherichia coli | |
| 0.0435 | - |
tRNACys | mutant enzyme E354A, mutant tRNACys(G15-C48) | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P21888 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-cysteine + tRNACys | - |
Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
tRNACys | mutant enzyme N351D, mutant tRNACys(G15-C48) | Escherichia coli | |
| 0.036 | - |
tRNACys | mutant enzyme E354A, mutant tRNACys(G15-C48) | Escherichia coli | |
| 0.074 | - |
tRNACys | mutant enzyme E354Q, mutant tRNACys(G15-C48) | Escherichia coli | |
| 0.094 | - |
tRNACys | mutant enzyme N351D, wild-type tRNACys(G15-G48) | Escherichia coli | |
| 0.1 | - |
tRNACys | mutant enzyme E354A, wild-type tRNACys(G15-G48) | Escherichia coli | |
| 0.14 | - |
tRNACys | mutant enzyme E354Q, wild-type tRNACys(G15-G48) | Escherichia coli | |
| 0.39 | - |
tRNACys | mutant enzyme N351A, mutant tRNACys(G15-C48) | Escherichia coli | |
| 0.45 | - |
tRNACys | wild-type enzyme, mutant tRNACys(G15-C48) | Escherichia coli | |
| 0.59 | - |
tRNACys | mutant enzyme N351A, wild-type tRNACys(G15-G48) | Escherichia coli | |
| 2.46 | - |
tRNACys | wild-type enzyme, wild-type tRNACys(G15-G48) | Escherichia coli | |
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