Protein Variants | Comment | Organism |
---|---|---|
V27E | mutation does not affect the discrimination of the enzyme for serine. 4fold increase in Km-value for cysteine and 9fold reduction of turnover number for ATP | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00064 | - |
tRNACys | 30°C, pH 7.0, wild-type enzyme | Escherichia coli | |
0.00092 | - |
tRNACys | 30°C, pH 7.0, mutant enzyme V27E | Escherichia coli | |
0.0072 | - |
L-cysteine | 30°C, pH 7.0, wild-type enzyme | Escherichia coli | |
0.0282 | - |
L-cysteine | 30°C, pH 7.0, mutant enzyme V27E | Escherichia coli | |
0.338 | - |
ATP | 30°C, pH 7.0, wild-type enzyme | Escherichia coli | |
0.412 | - |
ATP | 30°C, pH 7.0, mutant enzyme V27E | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | - |
Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
ATP | 30°C, pH 7.0, mutant enzyme V27E | Escherichia coli | |
0.68 | - |
tRNACys | 30°C, pH 7.0, mutant enzyme V27E | Escherichia coli | |
0.86 | - |
L-cysteine | 30°C, pH 7.0, mutant enzyme V27E | Escherichia coli | |
2.9 | - |
tRNACys | 30°C, pH 7.0, wild-type enzyme | Escherichia coli | |
4.4 | - |
ATP | 30°C, pH 7.0, wild-type enzyme | Escherichia coli | |
4.8 | - |
L-cysteine | 30°C, pH 7.0, wild-type enzyme | Escherichia coli |