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Literature summary for 6.1.1.16 extracted from

  • Stathopoulos, C.; Kim, W.; Li, T.; Anderson, I.; Deutsch, B.; Palioura, S.; Whitman, W.; Soll, D.
    Cysteinyl-tRNA synthetase is not essential for viability of the archaeon Methanococcus maripaludis (2001), Proc. Natl. Acad. Sci. USA, 98, 14292-14297.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.16

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the wild-type enzyme in Escherichia coli BL21(DE3) as N-terminally HIs-tagged protein Methanococcus maripaludis

Protein Variants

Protein Variants Comment Organism
additional information construction of a cysS gene disruption knockout mutant, which is viable under normal growth conditions Methanococcus maripaludis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0097
-
 L-cysteine recombinant wild-type enzyme, pH 7.0, 37°C Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-cysteine + tRNACys Methanococcus maripaludis the canonical enzyme is not essential for viability of the archaeon Methanococcus maripaludis probably due to the dual-specific ProCys-tRNA synthetase AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Organism

Organism UniProt Comment Textmining
Methanococcus maripaludis Q9UWP2 gene cysS, canonical enzyme form CysRS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNACys
-
 Methanococcus maripaludis AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
ATP + L-cysteine + tRNACys the canonical enzyme is not essential for viability of the archaeon Methanococcus maripaludis probably due to the dual-specific ProCys-tRNA synthetase Methanococcus maripaludis AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
additional information the enzyme also performs the ATP-diphosphate exchange reaction Methanococcus maripaludis ?
-
 ?

Synonyms

Synonyms Comment Organism
CysRS
-
 Methanococcus maripaludis
Cysteine translase
-
 Methanococcus maripaludis
Cysteine--tRNA ligase
-
 Methanococcus maripaludis
Cysteinyl-transfer ribonucleate synthetase
-
 Methanococcus maripaludis
Cysteinyl-transfer RNA synthetase
-
 Methanococcus maripaludis
Cysteinyl-tRNA synthetase
-
 Methanococcus maripaludis
Synthetase, cysteinyl-transfer ribonucleate
-
 Methanococcus maripaludis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanococcus maripaludis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2
-
 L-cysteine recombinant wild-type enzyme, pH 7.0, 37°C Methanococcus maripaludis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methanococcus maripaludis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanococcus maripaludis