Cloned (Comment) | Organism |
---|---|
overexpression of the wild-type enzyme in Escherichia coli BL21(DE3) as N-terminally HIs-tagged protein | Methanococcus maripaludis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a cysS gene disruption knockout mutant, which is viable under normal growth conditions | Methanococcus maripaludis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0097 | - |
L-cysteine | recombinant wild-type enzyme, pH 7.0, 37°C | Methanococcus maripaludis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | Methanococcus maripaludis | the canonical enzyme is not essential for viability of the archaeon Methanococcus maripaludis probably due to the dual-specific ProCys-tRNA synthetase | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus maripaludis | Q9UWP2 | gene cysS, canonical enzyme form CysRS | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | - |
Methanococcus maripaludis | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
ATP + L-cysteine + tRNACys | the canonical enzyme is not essential for viability of the archaeon Methanococcus maripaludis probably due to the dual-specific ProCys-tRNA synthetase | Methanococcus maripaludis | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
additional information | the enzyme also performs the ATP-diphosphate exchange reaction | Methanococcus maripaludis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CysRS | - |
Methanococcus maripaludis |
Cysteine translase | - |
Methanococcus maripaludis |
Cysteine--tRNA ligase | - |
Methanococcus maripaludis |
Cysteinyl-transfer ribonucleate synthetase | - |
Methanococcus maripaludis |
Cysteinyl-transfer RNA synthetase | - |
Methanococcus maripaludis |
Cysteinyl-tRNA synthetase | - |
Methanococcus maripaludis |
Synthetase, cysteinyl-transfer ribonucleate | - |
Methanococcus maripaludis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanococcus maripaludis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
L-cysteine | recombinant wild-type enzyme, pH 7.0, 37°C | Methanococcus maripaludis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanococcus maripaludis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanococcus maripaludis |