Cloned (Comment) | Organism |
---|---|
overexpression in strain JM109 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | tRNAGln mutant substrates | Escherichia coli | |
0.0015 | - |
tRNACys | pH 7.0, 37°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | Escherichia coli | - |
AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from overexpressing strain JM109 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | - |
Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
ATP + L-cysteine + tRNACys mutant | tRNA substrate is a tRNACys with mutation at the core tertiary Levitt pair from wild-type G15.C48 to mutant G15.G48, leading to a higher activity | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys mutant | - |
? | |
ATP + L-cysteine + tRNAGln duoble-mutant | tRNA substrate is a tRNAGln with introduced tRNACys indentitiy nucleotides at the acceptor and anticodon ends and a core tertiary Levitt pair equivalent to tRNAGln of G15.G48, poor activity | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNAGln double-mutant | - |
? | |
ATP + L-cysteine + tRNAGln mutant | tRNA substrate is a tRNAGln with introduced tRNACys indentitiy nucleotides at the acceptor and anticodon ends and a core tertiary Levitt pair equivalent to tRNACys of G15.C48 | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNAGln mutant | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CysRS | - |
Escherichia coli |
Cysteine translase | - |
Escherichia coli |
Cysteine--tRNA ligase | - |
Escherichia coli |
Cysteinyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Cysteinyl-transfer RNA synthetase | - |
Escherichia coli |
Cysteinyl-tRNA synthetase | - |
Escherichia coli |
Synthetase, cysteinyl-transfer ribonucleate | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | tRNAGln mutant substrates | Escherichia coli | |
3 | 6 | tRNACys | pH 7.0, 37°C | Escherichia coli | |
3.47 | - |
tRNACys | pH 7.0, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |