KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
L-cysteine | pH 7.5, 25°C | Oryctolagus cuniculus | |
0.08 | - |
tRNACys | pH 7.5, 25°C | Oryctolagus cuniculus | |
0.8 | - |
ATP | pH 7.5, 25°C | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | required, optimal cocentration is 150 mM | Oryctolagus cuniculus | |
Mg2+ | dependent on, optimal concentration is 5 mM | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
x * 92000, enzyme form I, SDS-PAGE, x * 86000, enzyme form II, SDS-PAGE | Oryctolagus cuniculus |
92000 | - |
x * 92000, enzyme form I, SDS-PAGE, x * 86000, enzyme form II, SDS-PAGE | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | Oryctolagus cuniculus | - |
AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
8300fold from liver, 2 different forms, to homogeneity | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.22 | - |
purified enzyme | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 1 mM DTE, at least 6 months, no loss of activity | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | - |
Oryctolagus cuniculus | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 92000, enzyme form I, SDS-PAGE, x * 86000, enzyme form II, SDS-PAGE | Oryctolagus cuniculus |
Synonyms | Comment | Organism |
---|---|---|
CRS | - |
Oryctolagus cuniculus |
CysRS | - |
Oryctolagus cuniculus |
Cysteine translase | - |
Oryctolagus cuniculus |
Cysteine--tRNA ligase | - |
Oryctolagus cuniculus |
Cysteinyl-transfer ribonucleate synthetase | - |
Oryctolagus cuniculus |
Cysteinyl-transfer RNA synthetase | - |
Oryctolagus cuniculus |
Cysteinyl-tRNA synthetase | - |
Oryctolagus cuniculus |
Synthetase, cysteinyl-transfer ribonucleate | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on, optimal concentration is 3 mM | Oryctolagus cuniculus |