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Literature summary for 6.1.1.15 extracted from

  • Ravishankar, S.; Ambady, A.; Swetha, R.G.; Anbarasu, A.; Ramaiah, S.; Sambandamurthy, V.K.
    Essentiality assessment of cysteinyl and lysyl-tRNA synthetases of Mycobacterium smegmatis (2016), PLoS ONE, 11, e0147188 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.15

Application

Application Comment Organism
drug development aminoacyl-tRNA synthetases are attractive targets for the discovery of antibacterial agents Mycolicibacterium smegmatis

Cloned(Commentary)

Cloned (Comment) Organism
gene proS, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in conditional expression strains of Mycobacterium smegmatis strain mc2155 from pAZI9452 vector Mycolicibacterium smegmatis

Protein Variants

Protein Variants Comment Organism
additional information generation of conditional expression strains, conditional expression plasmids are electroporated into Mycobacterium smegmatis strain mc2155, analysis of inducer dependency of conditional expression strains, overview Mycolicibacterium smegmatis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane transmembrane segment analysis of lysyl-tRNA synthetase Mycolicibacterium smegmatis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-proline + tRNAPro Mycolicibacterium smegmatis
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro Mycolicibacterium smegmatis mc2155 / ATCC 700084
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0QVM0 gene proS
-
Mycolicibacterium smegmatis mc2155 / ATCC 700084 A0QVM0 gene proS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-proline + tRNAPro
-
 Mycolicibacterium smegmatis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro
-
 Mycolicibacterium smegmatis mc2155 / ATCC 700084 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Subunits

Subunits Comment Organism
More the enzyme possess motifs for a catalytic, an anti-codon binding and a tRNA editing activity, similar to the ones found in the canonical prolyl-tRNA synthetase of Escherichia coli and Mycobacterium tuberculosis Mycolicibacterium smegmatis

Synonyms

Synonyms Comment Organism
MSMEG_2621
-
 Mycolicibacterium smegmatis
Prolyl-tRNA synthetase
-
 Mycolicibacterium smegmatis
ProRS
-
 Mycolicibacterium smegmatis
proS
-
 Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mycolicibacterium smegmatis

General Information

General Information Comment Organism
evolution multiple genes encode for aminoacyl-tRNA synthetases from Mycobacterium smegmatis, e.g. glutamyl (GluRS), cysteinyl (CysRS), prolyl (ProRS) and lysyl (LysRS) tRNA synthetases. The enzyme possess motifs for a catalytic, an anti-codon binding and a tRNA editing activity, similar to the ones found in the canonical prolyl-tRNA synthetase of Escherichia coli and Mycobacterium tuberculosis Mycolicibacterium smegmatis
additional information sequence and structural analysis of prolyl-tRNA synthetase, overview Mycolicibacterium smegmatis