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Literature summary for 6.1.1.15 extracted from

  • Larson, E.T.; Kim, J.E.; Napuli, A.J.; Verlinde, C.L.; Fan, E.; Zucker, F.H.; Van Voorhis, W.C.; Buckner, F.S.; Hol, W.G.; Merritt, E.A.
    Structure of the prolyl-tRNA synthetase from the eukaryotic pathogen Giardia lamblia (2012), Acta Crystallogr. Sect. D, 68, 1194-1200.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Giardia intestinalis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 28% (w/v) PEG 3350, 0.2 M magnesium chloride, 0.1 M Tris-HCl pH 7.8, at 4°C Giardia intestinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-proline + tRNAPro Giardia intestinalis
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro Giardia intestinalis WB / CC 30957
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Organism

Organism UniProt Comment Textmining
Giardia intestinalis A8BR89 synonym Giardia lamblia
-
Giardia intestinalis WB / CC 30957 A8BR89 synonym Giardia lamblia
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-proline + tRNAPro
-
 Giardia intestinalis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro
-
 Giardia intestinalis WB / CC 30957 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Synonyms

Synonyms Comment Organism
Prolyl-tRNA synthetase
-
 Giardia intestinalis
ProRS
-
 Giardia intestinalis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Giardia intestinalis