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Literature summary for 6.1.1.15 extracted from

  • Cusack, S.; Yaremchuk, A.; Krikliviy, I.; Tukalo, M.
    tRNAPro anticodon recognition by Thermus thermophilus prolyl-tRNA synthetase (1998), Structure, 6, 101-108.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified free enzyme or complexed with 3 tRNAPro isoacceptors, precipitant is 32% ammonium sulfate at pH 7.5, X-ray diffraction structure determination at 3.5 A resolution, and analysis Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-proline + tRNAPro Thermus thermophilus
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
purified class IIa enzyme
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
purified class IIa enzyme
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro substrate binding mechanism, the two bases G35 and G36 are sufficient to uniquely identify the cognate tRNAPro Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-proline + tRNAPro
-
Thermus thermophilus AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro the C-terminal anticodon binding domain with an alpha/beta fold binds to the anticodon stem-loop from the major groove side Thermus thermophilus AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro the C-terminal anticodon binding domain with an alpha/beta fold binds to the anticodon stem-loop from the major groove side Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-prolyl-tRNAPro
-
?

Synonyms

Synonyms Comment Organism
Global RNA synthesis factor
-
Thermus thermophilus
Pro-tRNA synthetase
-
Thermus thermophilus
Proline translase
-
Thermus thermophilus
Proline--tRNA ligase
-
Thermus thermophilus
Prolyl RNA synthetase
-
Thermus thermophilus
Prolyl-transfer ribonucleate synthetase
-
Thermus thermophilus
Prolyl-transfer ribonucleic acid synthetase
-
Thermus thermophilus
Prolyl-transfer RNA synthetase
-
Thermus thermophilus
Prolyl-tRNA synthetase
-
Thermus thermophilus
ProRS
-
Thermus thermophilus
ProRSTT
-
Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Thermus thermophilus