Crystallization (Comment) | Organism |
---|---|
purified free enzyme or complexed with 3 tRNAPro isoacceptors, precipitant is 32% ammonium sulfate at pH 7.5, X-ray diffraction structure determination at 3.5 A resolution, and analysis | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | Thermus thermophilus | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
purified class IIa enzyme | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
purified class IIa enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro | substrate binding mechanism, the two bases G35 and G36 are sufficient to uniquely identify the cognate tRNAPro | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | - |
Thermus thermophilus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | the C-terminal anticodon binding domain with an alpha/beta fold binds to the anticodon stem-loop from the major groove side | Thermus thermophilus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | the C-terminal anticodon binding domain with an alpha/beta fold binds to the anticodon stem-loop from the major groove side | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Global RNA synthesis factor | - |
Thermus thermophilus |
Pro-tRNA synthetase | - |
Thermus thermophilus |
Proline translase | - |
Thermus thermophilus |
Proline--tRNA ligase | - |
Thermus thermophilus |
Prolyl RNA synthetase | - |
Thermus thermophilus |
Prolyl-transfer ribonucleate synthetase | - |
Thermus thermophilus |
Prolyl-transfer ribonucleic acid synthetase | - |
Thermus thermophilus |
Prolyl-transfer RNA synthetase | - |
Thermus thermophilus |
Prolyl-tRNA synthetase | - |
Thermus thermophilus |
ProRS | - |
Thermus thermophilus |
ProRSTT | - |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus |