Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanococcus maripaludis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
L-proline | pH 7.0, 37°C | Methanococcus maripaludis | |
0.0745 | - |
L-cysteine | pH 7.0, 37°C | Methanococcus maripaludis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | Methanococcus maripaludis | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus maripaludis | Q6LZD3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanococcus maripaludis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNA | in later work it is shown that cysteine is attached to tRNA(Pro) and thus constitutes a misaminoacylation event and not a dual specificity | Methanococcus maripaludis | AMP + diphosphate + L-cysteinyl-tRNA | - |
? | |
ATP + L-proline + tRNAPro | - |
Methanococcus maripaludis | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dual-specificity prolyl-tRNA synthetase | misleading | Methanococcus maripaludis |
ProCysRS | - |
Methanococcus maripaludis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanococcus maripaludis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
L-cysteine | pH 7.0, 37°C | Methanococcus maripaludis | |
0.9 | - |
L-proline | pH 7.0, 37°C | Methanococcus maripaludis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanococcus maripaludis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.7 | - |
L-cysteine | pH 7.0, 37°C | Methanococcus maripaludis | |
196 | - |
L-proline | pH 7.0, 37°C | Methanococcus maripaludis |