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Literature summary for 6.1.1.15 extracted from

  • Peterson, P.J.; Fowden, L.
    Purification, properties and comparative specificities of the enzyme prolyl-transfer ribonucleic acid synthetase from Phaseolus aureus and Polygonatum multiflorum (1965), Biochem. J., 97, 112-124.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.15

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate
-
 Phaseolus vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Phaseolus vulgaris
Mg2+ required Polygonatum multiflorum
Mn2+ can partially replace Mg2+ in activation Phaseolus vulgaris
Mn2+ can partially replace Mg2+ in activation Polygonatum multiflorum

Organism

Organism UniProt Comment Textmining
Asparagus officinalis
-
-
-
Convallaria majalis
-
-
-
Phaseolus vulgaris
-
-
-
Pisum sativum
-
-
-
Polygonatum multiflorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phaseolus vulgaris
-
 Polygonatum multiflorum

Source Tissue

Source Tissue Comment Organism Textmining
rhizome apices Convallaria majalis
-
rhizome apices Polygonatum multiflorum
-
seed
-
 Phaseolus vulgaris
-
seed
-
 Convallaria majalis
-
seed
-
 Pisum sativum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-proline + tRNAPro
-
 Polygonatum multiflorum AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) Phaseolus vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) Asparagus officinalis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) Pisum sativum AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Phaseolus vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Convallaria majalis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange Phaseolus vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
62
-
 50% loss of activity after 5 min in Tris-HCl buffer Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
 Phaseolus vulgaris
8.4
-
-
 Polygonatum multiflorum