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Literature summary for 6.1.1.15 extracted from

  • Norton, S.J.
    Purification and properties of the prolyl RNA synthetase of Escherichia coli (1964), Arch. Biochem. Biophys., 106, 147-152.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
3,4-Dehydroproline competitive inhibition of proline transfer to tRNA, no inhibition of proline-dependent ATP-diphosphate exchange Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
Pro
-
Escherichia coli
0.6
-
ATP
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli 9723
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-proline + tRNAPro 4-hydroxy-L-proline can replace proline in ATP-diphosphate exchange Escherichia coli AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Escherichia coli AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro 4-hydroxy-L-proline can replace proline in ATP-diphosphate exchange Escherichia coli 9723 AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Escherichia coli 9723 AMP + diphosphate + L-prolyl-tRNAPro
-
?

pH Range

pH Minimum pH Maximum Comment Organism
6.8 7.6 variation of the pH in this range has little effect on activity Escherichia coli