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Literature summary for 6.1.1.14 extracted from
- Cocrystal structures of glycyl-tRNA synthetase in complex with tRNA suggest multiple conformational states in glycylation (2014), J. Biol. Chem., 289, 20359-20369 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| vapor diffusion method, using 0.2% (w/v) 4-diaminobutane, 0.2% (w/v) cystamine dihydrochloride, 0.2% (w/v) diloxanide furoate, 0.2% (w/v) sarcosine, 0.2% (w/v) spermine, and 0.02 M HEPES sodium (pH 6.8) at a 2:1:1 ratio | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E71G | the mutant shows increased activity compared to the wild type enzyme | Homo sapiens |
| Q640A | the mutation reduces the enzymatic activity by more than 10fold | Homo sapiens |
| Q675A | the mutation reduces the enzymatic activity by more than 10fold | Homo sapiens |
| Q675N | the mutation reduces the enzymatic activity by more than 10fold | Homo sapiens |
| Q82N | the mutant shows slightly increased activity compared to the wild type enzyme | Homo sapiens |
| R283A | the mutants retain 2% glycylation activity compared to the wild type enzyme | Homo sapiens |
| R283K | the mutants retain 20% glycylation activity compared to the wild type enzyme | Homo sapiens |
| R548A | the mutant shows strongly reduced activity compared to the wild type enzyme | Homo sapiens |
| R67A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
| S281A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
| S91A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
| T617A | the mutation reduces the enzymatic activity by more than 10fold | Homo sapiens |
| T631A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
| Y604F | the mutant shows strongly reduced activity compared to the wild type enzyme | Homo sapiens |
| Y604F | the mutation reduces the enzymatic activity by more than 10fold | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycine + tRNAGly | Homo sapiens | - |
AMP + diphosphate + glycyl-tRNAGly | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P41250 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Superdex 200 gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycine + tRNAGly | - |
Homo sapiens | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glycyl-tRNA synthetase | - |
Homo sapiens |
| GlyRS | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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