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Literature summary for 6.1.1.14 extracted from

  • Wu, Y.H.; Chang, C.P.; Chien, C.I.; Tseng, Y.K.; Wang, C.C.
    An insertion peptide in yeast glycyl-tRNA synthetase facilitates both productive docking and catalysis of cognate tRNAs (2013), Mol. Cell. Biol., 33, 3515-3523.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain InvSc1 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00028
-
 tRNAGly wild type isoform GlyRS1, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae
0.00073
-
 tRNAGly wild type isoform GlyRS2, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae
0.00137
-
 tRNAGly wild type isoform GlyRS1 without insertion peptide, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae
0.13
-
 glycine wild type isoform GlyRS1(KMM1), in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae
0.135
-
 glycine wild type isoform GlyRS1, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae
0.14
-
 glycine wild type isoform GlyRS1 without K motif, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae
0.145
-
 glycine wild type isoform GlyRS1 without insertion peptide, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae
0.145
-
 glycine wild type isoform GlyRS2, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Saccharomyces cerevisiae 5737
-
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glycine + tRNAGly Saccharomyces cerevisiae
-
 AMP + diphosphate + glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly Saccharomyces cerevisiae ATCC 204508
-
 AMP + diphosphate + glycyl-tRNAGly
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P38088 mitochondrial isoform GlyRS1
-
Saccharomyces cerevisiae Q06817 isoform GlyRS2
-
Saccharomyces cerevisiae ATCC 204508 P38088 mitochondrial isoform GlyRS1
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycine + tRNAGly
-
 Saccharomyces cerevisiae AMP + diphosphate + glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly
-
 Saccharomyces cerevisiae ATCC 204508 AMP + diphosphate + glycyl-tRNAGly
-
 ?

Synonyms

Synonyms Comment Organism
Glycyl-tRNA synthetase
-
 Saccharomyces cerevisiae
GlyRS
-
 Saccharomyces cerevisiae
GlyRS1 isoform Saccharomyces cerevisiae
GlyRS2 isoform Saccharomyces cerevisiae
GRS1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.19
-
 tRNAGly wild type isoform GlyRS1 without insertion peptide, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae
0.33
-
 tRNAGly wild type isoform GlyRS1, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae
0.73
-
 tRNAGly wild type isoform GlyRS2, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
140
-
 tRNAGly wild type isoform GlyRS1 without insertion peptide, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae
220
-
 tRNAGly wild type isoform GlyRS2, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae
1230
-
 tRNAGly wild type isoform GlyRS1, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C Saccharomyces cerevisiae