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Literature summary for 6.1.1.14 extracted from
- The crystal structures of the alpha-subunit of the alpha2beta2 tetrameric glycyl-tRNA synthetase (2012), J. Struct. Funct. Genomics, 13, 233-239.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Campylobacter jejuni subsp. jejuni |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| alpha-subunit the enzyme and its complexes with ATP, and ATP and glycine, sitting drop vapor diffusion method, using 0.1 M Bis-Tris propane:NaOH pH 7.0 and 0.7 M magnesium formate (complex with glycine), or 0.1 M Tris-HCl pH 8.5 and 0.3 M magnesium chloride (complex with ATP), or 0.1 M Na2HPO4-citrate acid pH 4.2, 0.2 M lithium sulfate and 20% (w/v) PEG 1000 (complex with ATP and glycine) | Campylobacter jejuni subsp. jejuni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycine + tRNAGly | Campylobacter jejuni subsp. jejuni | - |
AMP + diphosphate + glycyl-tRNAGly | - |
? |  |
| ATP + glycine + tRNAGly | Campylobacter jejuni subsp. jejuni NCTC 11168 | - |
AMP + diphosphate + glycyl-tRNAGly | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Campylobacter jejuni subsp. jejuni | Q9PPK3 | alpha subunit | - |
| Campylobacter jejuni subsp. jejuni NCTC 11168 | Q9PPK3 | alpha subunit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-affinity column chromatography | Campylobacter jejuni subsp. jejuni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycine + tRNAGly | - |
Campylobacter jejuni subsp. jejuni | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
| ATP + glycine + tRNAGly | - |
Campylobacter jejuni subsp. jejuni NCTC 11168 | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | - |
Campylobacter jejuni subsp. jejuni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glycyl-tRNA synthetase | - |
Campylobacter jejuni subsp. jejuni |
| GlyRS | - |
Campylobacter jejuni subsp. jejuni |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Campylobacter jejuni subsp. jejuni | |
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Release 2023.1 (January 2023)
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