Cloned (Comment) | Organism |
---|---|
overexpression of isozyme GlyRS1 in Escherichia coli strain TG2 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme GlyRS2 | Phaseolus vulgaris | 9507 | - |
chloroplast | isozyme GlyRS2 | Arabidopsis thaliana | 9507 | - |
cytosol | isozyme GlyRS1, active | Phaseolus vulgaris | 5829 | - |
cytosol | isozyme GlyRS1, active | Arabidopsis thaliana | 5829 | - |
mitochondrion | isozyme GlyRS2, enzyme is imported from the cytosol, nucleus-encoded | Arabidopsis thaliana | 5739 | - |
mitochondrion | isozymes GlyRS1 and GlyRS2, enzymes are imported from the cytosol, nucleus-encoded, isozyme GlyRS1 is inactive in the mitochondria | Phaseolus vulgaris | 5739 | - |
mitochondrion | isozymes GlyRS1, enzyme is imported from the cytosol, nucleus-encoded, isozyme GlyRS1 is inactive in the mitochondria | Arabidopsis thaliana | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
2-3 * 27000, about, partially processed isozyme GlyRS1, SDS-PAGE | Arabidopsis thaliana |
30000 | - |
4 * 30000, about, partially processed isozyme GlyRS2, SDS-PAGE | Arabidopsis thaliana |
80000 | - |
isozyme GlyRS1 | Arabidopsis thaliana |
117000 | - |
isozyme GlyRS2 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | Phaseolus vulgaris | - |
AMP + diphosphate + glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | Arabidopsis thaliana | - |
AMP + diphosphate + glycyl-tRNAGly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O23627 | 2 dual-targeted isozymes | - |
Arabidopsis thaliana | Q8L785 | 2 dual-targeted isozymes | - |
Phaseolus vulgaris | - |
2 dual-targeted isozymes | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme GlyRS1 from Escherichia coli | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | - |
Phaseolus vulgaris | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | - |
Arabidopsis thaliana | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | chloroplastic and mitochondrial tRNA substrate specificity of chloroplastic isozyme GlyRS2, isozyme GlyRS1 is much less efficient | Phaseolus vulgaris | AMP + diphosphate + glycyl-tRNAGly | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer or trimer | 2-3 * 27000, about, partially processed isozyme GlyRS1, SDS-PAGE | Arabidopsis thaliana |
tetramer | 4 * 30000, about, partially processed isozyme GlyRS2, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
Glycine--tRNA ligase | - |
Phaseolus vulgaris |
Glycine--tRNA ligase | - |
Arabidopsis thaliana |
Glycyl translase | - |
Phaseolus vulgaris |
Glycyl translase | - |
Arabidopsis thaliana |
Glycyl-transfer ribonucleate synthetase | - |
Phaseolus vulgaris |
Glycyl-transfer ribonucleate synthetase | - |
Arabidopsis thaliana |
Glycyl-transfer ribonucleic acid synthetase | - |
Phaseolus vulgaris |
Glycyl-transfer ribonucleic acid synthetase | - |
Arabidopsis thaliana |
Glycyl-transfer RNA synthetase | - |
Phaseolus vulgaris |
Glycyl-transfer RNA synthetase | - |
Arabidopsis thaliana |
Glycyl-tRNA synthetase | - |
Phaseolus vulgaris |
Glycyl-tRNA synthetase | - |
Arabidopsis thaliana |
GlyRS | - |
Phaseolus vulgaris |
GlyRS | - |
Arabidopsis thaliana |
Synthetase, glycyl-transfer ribonucleate | - |
Phaseolus vulgaris |
Synthetase, glycyl-transfer ribonucleate | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Phaseolus vulgaris | |
ATP | - |
Arabidopsis thaliana |