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Literature summary for 6.1.1.14 extracted from

  • Mazauric, M.H.; Keith, G.; Logan, D.; Kreutzer, R.; Giege, R.; Kern, D.
    Glycyl-tRNA synthetase from Thermus thermophilus--wide structural divergence with other prokaryotic glycyl-tRNA synthetases and functional inter-relation with prokaryotic and eukaryotic glycylation systems (1998), Eur. J. Biochem., 251, 744-757.
    View publication on PubMed
Search for more literature for 6.1.1.14

Cloned(Commentary)

Cloned (Comment) Organism
gene glyS, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli BL21(DE3) Thermus thermophilus

General Stability

General Stability Organism
glycyl-adenylate stabilizes the enzyme Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics for tRNAGly substrates of diverse prokaryotic and eukaryotic origins Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 x * 51000, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glycine + tRNAGly Thermus thermophilus
-
 AMP + diphosphate + glycyl-tRNAGly
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
 class II enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli, 10fold Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.124
-
 purified recombinant enzyme Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycine + tRNAGly
-
 Thermus thermophilus AMP + diphosphate + glycyl-tRNAGly
-
 ?
ATP + glycine + tRNAGly substrate specificity with tRNAGly of diverse origins Thermus thermophilus AMP + diphosphate + glycyl-tRNAGly
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE Thermus thermophilus
More comparison of structures of enzymes of diverse origins Thermus thermophilus

Synonyms

Synonyms Comment Organism
Glycine--tRNA ligase
-
 Thermus thermophilus
Glycyl translase
-
 Thermus thermophilus
Glycyl-transfer ribonucleate synthetase
-
 Thermus thermophilus
Glycyl-transfer ribonucleic acid synthetase
-
 Thermus thermophilus
Glycyl-transfer RNA synthetase
-
 Thermus thermophilus
Glycyl-tRNA synthetase
-
 Thermus thermophilus
GlyRS
-
 Thermus thermophilus
Synthetase, glycyl-transfer ribonucleate
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information kcat for tRNAGly substrates of diverse prokaryotic and eukaryotic origins Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Thermus thermophilus