Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 7% (w/v) PEG 4000, 0.1 M ammonium sulfate, 0.1 M sodium citrate pH 5.6 | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | Helicobacter pylori | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | Helicobacter pylori ATCC 700392 | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | P56459 | - |
- |
Helicobacter pylori ATCC 700392 | P56459 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and Sephacryl S200 gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | - |
Helicobacter pylori | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Helicobacter pylori ATCC 700392 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aspartyl-tRNA synthetase | - |
Helicobacter pylori |
AspRS | - |
Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Helicobacter pylori |