Literature summary for 6.1.1.12 extracted from

Bour, T.; Akaddar, A.; Lorber, B.; Blais, S.; Balg, C.; Candolfi, E.; Frugier, M.
Plasmodial aspartyl-tRNA synthetases and peculiarities in Plasmodium falciparum (2009), J. Biol. Chem., 284, 18893-18903.

Search for more literature for 6.1.1.12

Cloned(Commentary)

Cloned (Comment)	Organism
into the vector pGEX-2T for expression in Escherichia coli ER2566 cells	Homo sapiens
into the vector pGEX-2T for expression in Escherichia coli ER2566 cells	Plasmodium falciparum

Protein Variants

Protein Variants	Comment	Organism
additional information	the RNA binding motif is mutated, from 70KKKEKKAKK78 to 70QEEREEGQM78	Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
5'-O-[N-(L-aspartyl)sulfamoyl]adenosine	ASP-AMS	Homo sapiens
5'-O-[N-(L-aspartyl)sulfamoyl]adenosine	ASP-AMS	Plasmodium falciparum
L-aspartol-adenylate	aspartol-AMP	Homo sapiens
L-aspartol-adenylate	aspartol-AMP	Plasmodium falciparum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000018	-	tRNAAsp	Plasmodium falciparum wild type aspartyl-tRNA synthetase, amino acids 49-626	Plasmodium falciparum
0.00016	-	tRNAAsp	Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500	Homo sapiens
0.0024	-	tRNAAsp	Plasmodium falciparum aspartyl-tRNA synthetase, mutated RNA binding motif	Plasmodium falciparum
0.016	-	L-aspartate	Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500	Homo sapiens
0.14	-	ATP	Plasmodium falciparum wild type aspartyl-tRNA synthetase, amino acids 49-626	Plasmodium falciparum
0.21	-	L-aspartate	Plasmodium falciparum wild type aspartyl-tRNA synthetase, amino acids 49-626	Plasmodium falciparum
0.515	-	ATP	Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Homo sapiens	5737	-
cytosol	-	Plasmodium falciparum	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	determined by SDS-PAGE	Homo sapiens
64000	-	determined by SDS-PAGE	Saccharomyces cerevisiae
66000	-	determined by SDS-PAGE	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-aspartate + tRNAAsp	Homo sapiens	-	AMP + diphosphate + L-aspartyl-tRNAAsp	-	?
ATP + L-aspartate + tRNAAsp	Saccharomyces cerevisiae	-	AMP + diphosphate + L-aspartyl-tRNAAsp	-	?
ATP + L-aspartate + tRNAAsp	Plasmodium falciparum	-	AMP + diphosphate + L-aspartyl-tRNAAsp	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Plasmodium falciparum	Q8I2B1	-	-
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
on a glutathione-Sepharose resin	Homo sapiens
on a glutathione-Sepharose resin	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-aspartate + tRNAAsp	-	Homo sapiens	AMP + diphosphate + L-aspartyl-tRNAAsp	-	?
ATP + L-aspartate + tRNAAsp	-	Saccharomyces cerevisiae	AMP + diphosphate + L-aspartyl-tRNAAsp	-	?
ATP + L-aspartate + tRNAAsp	-	Plasmodium falciparum	AMP + diphosphate + L-aspartyl-tRNAAsp	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
Aspartyl-tRNA synthetase	-	Homo sapiens
Aspartyl-tRNA synthetase	-	Saccharomyces cerevisiae
Aspartyl-tRNA synthetase	-	Plasmodium falciparum
AspRS	-	Homo sapiens
AspRS	-	Saccharomyces cerevisiae
AspRS	-	Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	ATP/diphosphate exchange and tRNA aminoacylation assay	Homo sapiens
37	-	ATP/diphosphate exchange and tRNA aminoacylation assay	Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.01	-	tRNAAsp	Plasmodium falciparum aspartyl-tRNA synthetase, mutated RNA binding motif	Plasmodium falciparum
0.2	-	tRNAAsp	Plasmodium falciparum wild type aspartyl-tRNA synthetase, amino acids 49-626	Plasmodium falciparum
0.22	-	tRNAAsp	Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500	Homo sapiens
5.9	-	ATP	Plasmodium falciparum wild type aspartyl-tRNA synthetase, amino acids 49-626, ATP-diphosphate exchange	Plasmodium falciparum
6.7	-	ATP	Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500, ATP-diphosphate exchange	Homo sapiens
8.5	-	ATP	Plasmodium falciparum aspartyl-tRNA synthetase, mutated RNA binding motif, ATP-diphosphate exchange	Plasmodium falciparum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	ATP/diphosphate exchange and tRNA aminoacylation assay	Homo sapiens
7.4	-	ATP/diphosphate exchange and tRNA aminoacylation assay	Plasmodium falciparum

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens
ATP	-	Saccharomyces cerevisiae
ATP	-	Plasmodium falciparum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000079	-	5'-O-[N-(L-aspartyl)sulfamoyl]adenosine	Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500	Homo sapiens
0.00026	-	5'-O-[N-(L-aspartyl)sulfamoyl]adenosine	Plasmodium falciparum wild type aspartyl-tRNA synthetase, amino acids 49-626	Plasmodium falciparum
0.00033	-	L-aspartol-adenylate	Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500	Homo sapiens
0.0008	-	L-aspartol-adenylate	Plasmodium falciparum wild type aspartyl-tRNA synthetase, amino acids 49-626	Plasmodium falciparum

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Release 2023.1 (January 2023)