Cloned (Comment) | Organism |
---|---|
gene aspS, expression of the His-tagged enzyme in strain BL21 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K198L | site-directed mutagenesis, the mutant shows reduced ATP-diphosphate exchange activity compared to the wild-type enzyme | Escherichia coli |
Q199E | site-directed mutagenesis, the mutant shows reduced ATP-diphosphate exchange activity compared to the wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | ligand binding, kinetics and thermodynamics, wild-type and mutant enzymes, overview | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | Escherichia coli | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene aspS | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from strain BL21 by nickel affinity chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
ATP-diphosphate exchange activity of wild-type and mutant enzymes in the presence of Asp or Asn, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | - |
Escherichia coli | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
additional information | ATP-diphosphate exchange activity of wild-type and mutant enzymes in the presence of Asp or Asn, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aspartyl-tRNA synthetase | - |
Escherichia coli |
AspRS | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |