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Literature summary for 6.1.1.12 extracted from

  • Thompson, D.; Lazennec, C.; Plateau, P.; Simonson, T.
    Probing electrostatic interactions and ligand binding in aspartyl-tRNA synthetase through site-directed mutagenesis and computer simulations (2008), Proteins, 71, 1450-1460.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene aspS, expression of the His-tagged enzyme in strain BL21 Escherichia coli

Protein Variants

Protein Variants Comment Organism
K198L site-directed mutagenesis, the mutant shows reduced ATP-diphosphate exchange activity compared to the wild-type enzyme Escherichia coli
Q199E site-directed mutagenesis, the mutant shows reduced ATP-diphosphate exchange activity compared to the wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information ligand binding, kinetics and thermodynamics, wild-type and mutant enzymes, overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsp Escherichia coli
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene aspS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from strain BL21 by nickel affinity chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
ATP-diphosphate exchange activity of wild-type and mutant enzymes in the presence of Asp or Asn, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsp
-
Escherichia coli AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
additional information ATP-diphosphate exchange activity of wild-type and mutant enzymes in the presence of Asp or Asn, overview Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
Aspartyl-tRNA synthetase
-
Escherichia coli
AspRS
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli