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Literature summary for 6.1.1.12 extracted from
- Thermus thermophilus contains an eubacterial and an archaebacterial aspartyl-tRNA synthetase (2000), Biochemistry, 39, 3216-3230.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| aspS1 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha and BL21 | Thermus thermophilus |
| aspS2 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha and BL21 | Thermus thermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00003 | - |
tRNAAsp | pH 7.2, 70°C | Thermus thermophilus |  |
| 0.000063 | - |
tRNAAsn | pH 7.2, 70°C | Thermus thermophilus | |
| 0.000073 | - |
tRNAAsp | pH 7.2, 70°C | Thermus thermophilus | |
| 0.0034 | - |
tRNAAsn | pH 7.2, 70°C | Thermus thermophilus | |
| 0.005 | - |
L-aspartic acid | pH 7.2, 70°C | Thermus thermophilus | |
| 0.03 | - |
aspartic acid | pH 7.2, 70°C | Thermus thermophilus | |
| 0.033 | - |
ATP | pH 7.2, 70°C | Thermus thermophilus | |
| 0.28 | - |
ATP | pH 7.2, 70°C | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48329 | - |
x * 48329, amino acid sequence determination | Thermus thermophilus |
| 66029 | - |
x * 66029, amino acid sequence determination | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate + tRNAAsp | Thermus thermophilus | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| ATP + L-aspartate + tRNAAsp | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | P36419 | Asp-tRNA synthetase AspRS1 | - |
| Thermus thermophilus | Q5SIC2 | Asp-tRNA synthetase AspRS2 | - |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | P36419 | Asp-tRNA synthetase AspRS1 | - |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SIC2 | Asp-tRNA synthetase AspRS2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from overexpressing Escherichia coli, 34.3fold | Thermus thermophilus |
| recombinant from overexpressing Escherichia coli, 7.4fold | Thermus thermophilus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-aspartate + tRNAAsp = AMP + diphosphate + L-aspartyl-tRNAAsp | conserved amino acid residues of functional importance in consensus motifs: Pro227 in motif 1, Arg332 and Glu334 in motif 2, Gly675, Gly679, Asp681, Arg682, and Pro700 in motif 3 | Thermus thermophilus | |
| ATP + L-aspartate + tRNAAsp = AMP + diphosphate + L-aspartyl-tRNAAsp | conserved amino acid residues of functional importance in consensus motifs: Pro227 in motif 1, Arg332 and Glu334 in motif 2, Gly675, Gly679, Glu681, Arg682, and Pro700 in motif 3 | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00002 | - |
native enzyme, cell extract, 37°C | Thermus thermophilus |
| 0.000083 | - |
native enzyme, cell extract, 37°C | Thermus thermophilus |
| 0.0336 | - |
purified recombinant enzyme, 37°C | Thermus thermophilus |
| 0.043 | - |
recombinant enzyme, Escherichia coli DH5alpha cell extract, 37°C | Thermus thermophilus |
| 0.055 | - |
recombinant enzyme, Escherichia coli BL21 cell extract, 37°C | Thermus thermophilus |
| 0.124 | - |
recombinant enzyme, Escherichia coli BL21 cell extract, 37°C | Thermus thermophilus |
| 0.155 | - |
recombinant enzyme, Escherichia coli DH5alpha cell extract, 37°C | Thermus thermophilus |
| 0.321 | - |
purified recombinant enzyme, 37°C | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate + tRNAAsn | about half as effective as tRNAAsp | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| ATP + L-aspartate + tRNAAsn | about have as effective as tRNAAsp | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| ATP + L-aspartate + tRNAAsn | about half as effective as tRNAAsp | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| ATP + L-aspartate + tRNAAsn | about have as effective as tRNAAsp | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| ATP + L-aspartate + tRNAAsp | - |
Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| ATP + L-aspartate + tRNAAsp | Thermus thermophilus or Escherichia coli tRNA | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| ATP + L-aspartate + tRNAAsp | Thermus thermophilus or Eschrichia coli tRNA | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| ATP + L-aspartate + tRNAAsp | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| ATP + L-aspartate + tRNAAsp | Thermus thermophilus or Escherichia coli tRNA | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| ATP + L-aspartate + tRNAAsp | Thermus thermophilus or Eschrichia coli tRNA | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48329, amino acid sequence determination | Thermus thermophilus |
| ? | x * 66029, amino acid sequence determination | Thermus thermophilus |
| More | modular organization | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aspartic acid translase | - |
Thermus thermophilus |
| Aspartyl ribonucleate synthetase | - |
Thermus thermophilus |
| aspartyl ribonuleic synthetase | - |
Thermus thermophilus |
| Aspartyl-transfer ribonucleic acid synthetase | - |
Thermus thermophilus |
| Aspartyl-transfer RNA synthetase | - |
Thermus thermophilus |
| Aspartyl-tRNA synthetase | - |
Thermus thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.092 | - |
tRNAAsn | pH 7.2, 70°C | Thermus thermophilus | |
| 0.12 | - |
tRNAAsn | pH 7.2, 70°C | Thermus thermophilus | |
| 0.24 | - |
tRNAAsp | pH 7.2, 70°C | Thermus thermophilus | |
| 2.7 | - |
tRNAAsp | pH 7.2, 70°C | Thermus thermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Thermus thermophilus |
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Release 2023.1 (January 2023)
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