Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Br- | - |
Sus scrofa | |
CaCl2 | 1 mM, more than 50% inhibition | Sus scrofa | |
Cl- | - |
Sus scrofa | |
F- | - |
Sus scrofa | |
I- | - |
Sus scrofa | |
NiCl2 | 0.1 mM more than 50% inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000061 | - |
tRNAAsp | - |
Sus scrofa | |
0.067 | - |
Asp | - |
Sus scrofa | |
0.26 | - |
ATP | - |
Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Sus scrofa | |
Mg2+ | optimal Mg2+/ATP ratio is 2 | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, gel electrophoresis under denaturing conditions | Sus scrofa |
124000 | - |
gel filtration | Sus scrofa |
156000 | - |
nondenaturing PAGE | Sus scrofa |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | 25% v/v, required throughout purification procedure to maintain stability | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme form PC-1 (in addition 3 low MW enzyme forms exist: PC-2a, PC-2b and PC-2c) | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thyroid gland | - |
Sus scrofa | - |
Storage Stability | Organism |
---|---|
-20°C, 10 mM potassium phosphate, pH 7.6, 10 mM 2-mercaptoethanol, 50% glycerol, stable | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | - |
Sus scrofa | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, gel electrophoresis under denaturing conditions | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | potassium Hepes buffer | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NiCl2 | - |
Sus scrofa | |
1 | - |
CaCl2 | - |
Sus scrofa |